Synthesis of quaternary piperazine methacrylate homopolymers and their antibiofilm and anti‐quorum sensing effects on pathogenic bacteria

Abstract

In this work, poly(glycidyl methacrylate) (PGMA, G) homopolymers with different molecular weights (5600-21,000 gmol −1) were synthesized by atom transfer radical polymerization (ATRP) method. Epoxy groups of PGMA homopolymers were converted to hydroxyl and 1-methyl piperazin residues by reacting with 1-methyl piperazine via ring-opening reaction. The obtained poly(2-hydroxy-3-methyl piperazinepropyl methacrylate) (PHMPPMA, G-P) homopolymers (from 9450 to 35,900 gmol −1) were reacted with alkyl halides of different carbon chain lengths (methyl-, ethyl-, propyl-, butyl-, pentyl-iodides, hexyl bromide) and benzyl chloride (arylhalide) to form quaternary PHMPPMA homopolymers (G-QP). MIC values varied from 0.125 mg/ml to

Antibiofilm and Anti-Quorum Sensing Potential of Cycloartane-Type Triterpene Acids from Cameroonian Grassland Propolis: Phenolic Profile and Antioxidant Activity of Crude Extract

“…Terpenoids and phenolic compounds contained in natural products are usually responsible for the anti-QS activity, and all these classes are contained in propolis and propolis samples containing various phenolic and terpenoid components that have been reported for anti-QS activity, violacein inhibition, and swarming/swimming motilities inhibition [ 67 , 68 , 69 , 70 ]. Bacterial motility is a quorum sensing mediated process that involves swarming and swimming movements, powered by flagella in bacteria such as P. aeruginosa PA01, which enables them to move to and attach themselves on surfaces before colonizing the surfaces and establishing biofilms [ 9 , 71 , 72 ]. Inhibiting swimming and swarming motilities therefore can greatly reduce microbial resistance and the incidence of surface colonization and biofilm formation on surfaces in general and most especially on medical devices and implants in particular, and this can eradicate community-acquired and healthcare-associated infections.…”

Synthesis and evaluation of inhibitory potentials of microbial biofilms and quorum-sensing by 3-(1,3-dithian-2-ylidene) pentane-2,4-dione and ethyl-2-cyano-2-(1,3-dithian-2-ylidene) acetate

“…Also, since C. violaceum CV026 cannot produce AHL on its own, an external AHL was supply to it but zones of non-production of violacein were observed indicating that the compounds equally disrupted signal reception within the anti-QS zones (Tamfu et al 2020b). The inhibition of violacein production, quorum sensing, biofilm formation and swarming motility which are controlled by QS are suitable strategies of overcoming microbial resistance and a biocontrol method to reduce the spread of notorious infections and this can be done by using and some natural compounds, synthesized compounds, polymers, nanomaterials and natural products which have shown some promising effects (Ceylan et al 2020;Boudiba et al 2021;Kocak et al 2021;Popova et al 2021;Tamfu et al 2022c). Flagellum-mediated motility such as swarming movement serves the bacteria Pseudomonas aeruginosa PA01 to move to surface and attach themselves before further spreading and colonization of the surface (Ngenge Tamfu et al 2021).…”

“…Recent developments of strategies such as disruption of biofilm and QS are gaining grounds and are used to combat the emergence of resistant microbial strains which are continuously constituting a major health issue across the globe . There is a need for the search of alternative antimicrobial drugs which are safer with greater efficacy by re-searchers and this has motivated increasing investigations on herbal and synthetic products for the discovery of new anti-pathogenic therapeutics that might be less toxic and also capable of inhibiting QS, thereby reducing infections and at the same time controlling or avoiding development of resistance by bacteria (Tamfu et al 2020a;Ceylan et al 2020;Kocak et al 2021). The situation has created an urgent and highly demanding necessity for new antibiotic therapies that can subdue the emergence of strains that are resistant to existing antibiotics and disrupt microbial cellto-cell communication systems (QS) and elimination of biofilm formation which appears to be a suitable solution (Wang et al 2019).…”

Evaluation of Enzyme Inhibition and Anti-Quorum Sensing Potentials of Melaleuca alternifolia and Citrus sinensis Essential Oils

“…Pseudomonas aeruginosa PA01 utilizes flagellum-mediated motility to approach a surface, attaches, and then further spreads via the surface through associated motilities, such as swarming and twitching. 41,42 The swarming motility inhibition results indicate that C. sinensis oil is more active as it had a greater percentage inhibition than M. alternifolia oil at 100 and 75 µg/mL. Swarming motility is a favorable step toward the establishment of biofilms and colonization of surfaces by bacteria and therefore the ability of the oils to inhibit this swarming motility could reduce biofilm risks during infections.…”