Synthesis of porphyrins by cell suspensions of Tetrahymena vorax: effect of members of the vitamin B group

Abstract

in view of the finding that this compound readily undergoes rearrangement in alkaline solution to 16-oxo-oestradiol-17,B there can be little doubt that the KC-5 previously isolated by Marrian et al. (1957) was indeed a mixture of 16oc-hydroxyoestrone and artifactually produced 16-oxo-oestradiol-17 P as was The authors are grateful to the Medical Research Council for a grant from which the expenses of this work were defrayed. They are also grateful to their colleague Dr J. W. Minnis, who carried out all the microanalyses; to Dr T. E. Gallagher of the Sloan-Kettering Institute, New York, who generously provided a sample of authentic 16ax-hydroxyoestrone diacetate; to Dr R. K. Callow of the National Institute for Medical Research, who very kindly determined the infrared spectra; and to their colleagues in the Department of Obstetrics and Gynaecology and in the Clinical Endocrinology Research Unit (M.R.C.) who so willingly provided facilities for the collection of urine specimens. One of the authors (K. H. L.) is indebted to the Federation of Malaya for the award of a Queen's Scholarship.

Initial stages in the biosynthesis of porphyrins. 2. The formation of δ-aminolaevulic acid from glycine and succinyl-coenzyme A by particles from chicken erythrocytes

“…Shemin & Wittenberg (1951) suggested that it might be succinyl-coenzyme A (succinyl-CoA) even before the isolation of this substance by Sanadi & Littlefield (1953); but little supporting evidence was obtained for this idea. Strong, but indirect evidence that coenzyme A is involved in the synthesis of 8-aminolaevulic acid comes from the study of haem and porphyrin formation in pantothenate-deficient ducks (Schulinan & Richert, 1957) and Tetrahymena vorax (Lascelles, 1957). Coenzyme A produces also a stimulation of activity in the system studied by Laver et al (1958); but all attempts to determine its role in this reactionwere unsuccessful, and indeed no 8-aminolaevulic acid was formed when synthetic succinyl-coenzyme A was substituted for succinate or a-oxoglutarate.…”

Το Αιμοσφαιρικον Κλασμα Aic Επι Διαβητικων Ασθενων

“…Διά τόν σχηματισμόν της αΐμης, α-ό£υγλουταρικόν όξύ έκ τοϋ κύκλου του Krebs, ένοΰται μετά συνενζύμου Α καί σχηματίζεται ήλε κτρικός θειοεστήρ τοϋ συνενζύμου Α. Έν συνεχεία ò θειοεστήρ ούτος μετά γλυκίνης σχηματίζει, μέσω ήλεκτρογλυκίνης, δ-άμινολεβουλινικόν όξύ (δ-ALA) τη καταλυτική δράσει της συνθετάσης ö-ALA (Kappas καί συν. 1968, Lascelles 1963, Gibson καί συν. 1955.…”

Η Tetrahymena Pyriformis Σαν Βιοχημικο Συστημα Μελετης Των Δρασεων Και Της Παραγωγης Του Παραγοντα Ενεργοποιησης Αιμοπεταλιων

“…Τα λιποειδή και οι περισσότερες πρωτεΐνες παράγονται γιά εσωτερική χρήση του κυττάρου, ο πότε μπορούν και να ανιχνευθούν σ'αυτό. Η Τ,ργΓϊίοηπϊεδιαθέτετι τις μεταβολικές πορείες των θηλαστικών (2) και συγχρόνως βιοσυνθέτει πολύπλοκες, εξειδικευμένες ενώσεις όπως αιμοσφαι ρίνη, (19,20 ) > σεροτονίνη, (21,22 ), ισταμίνη, σωματοοτατίνη, κατεχολαμίυες, (23), β-ενδορφίνη (24,25), ρελαξίνη (26) και προσταγλανδίνες (27).…”