Synthesis of Polyampholyte Janus‐like Microgels by Coacervation of Reactive Precursors in Precipitation Polymerization

Xu, Wenjing; Rudov, Andrey A.; Oppermann, Alex; Wypysek, Sarah; Kather, Michael; Schroeder, Ricarda; Richtering, Walter; Wöll, Dominik; Pich, Andrij

doi:10.1002/anie.201910450

Cited by 25 publications

(17 citation statements)

References 56 publications

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“…A 3D cross-linked polymer colloids, nanogels (NGs) as nanocarriers have attracted much attention in biomedical applications, including drug/enzyme delivery, images and therapy of tumors, due to their unique properties [ [14] , [15] , [16] ]. All the time we explored various NGs that having the controllable size, deformable softness, highly porous network, excellent biocompatibility and stimuli-responsiveness can be employed as functional nanocarriers for improved tumor theranostics [ [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] ]. In particular, the biocompatible PVCL-based NGs exist a volume phase transition temperature (VPTT) of about 35 °C which is close to body temperature, and high cargo loading capacity along with enhanced tumor accumulation for biomedical purposes [ [26] , [27] , [28] ].…”

Section: Introductionmentioning

confidence: 99%

Dendrimer-decorated nanogels: Efficient nanocarriers for biodistribution in vivo and chemotherapy of ovarian carcinoma

Ouyang

et al. 2021

Bioactive Materials

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Nanomedicine has revolutionized disease theranostics by the accurate diagnosis and efficient therapy. Here, the PAMAM dendrimer decorated PVCL-GMA nanogels (NGs) were developed for favorable biodistribution in vivo and enhanced antitumor efficacy of ovarian carcinoma. By an ingenious design, the NGs with a unique structure that GMA-rich domains were localized on the surface were synthesized via precipitation polymerization. After G2 dendrimer decoration, the overall charge is changed from neutral to positive, and the NGs-G2 display the whole charge nature of positively charged corona and neutral core. Importantly, the unique architecture and charge conversion of NGs-G2 have a profound impact on the biodistribution and drug delivery in vivo . As a consequence of this alteration, the NGs-G2 as nanocarriers emerge the highly sought biodistribution of reduced liver accumulation, enhanced tumor uptake, and promoted drug release, resulting in the significantly augmented antitumor efficacy with low side effects. Remarkably, this finding is contrary to some reported work that the nanocarriers with positive charge have preferential liver uptake. Moreover, the NGs-G2 also displayed thermal/pH dual-responsive behaviors, excellent biocompatibility, improved cellular uptake, and stimuli-responsive drug release. Encouragingly, this work demonstrates a novel insight into the strategy for optimizing design, improving biodistribution and enhancing theranostic efficacy of nanocarriers.

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Section: Introductionmentioning

confidence: 99%

Dendrimer-decorated nanogels: Efficient nanocarriers for biodistribution in vivo and chemotherapy of ovarian carcinoma

Ouyang

et al. 2021

Bioactive Materials

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“…[30] Recent progress in synthesis enabled preparation nanogels with internal compartments and various shapes. [31][32][33][34][35] Furthermore,i sw as observed that the extreme softness of ultra-low cross-linked (ULC) nanogels leads to unique properties. [36] ULC nanogels exhibit high swellability and flexibility.…”

Section: Introductionmentioning

confidence: 99%

Stiffness Tomography of Ultra‐Soft Nanogels by Atomic Force Microscopy

et al. 2020

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The softness of nanohydrogels results in unique properties and recently attracted tremendous interest due to the multi‐functionalization of interfaces. Herein, we study extremely soft temperature‐sensitive ultra‐low cross‐linked (ULC) nanogels adsorbed to the solid/water interface by atomic force microscopy (AFM). The ultra‐soft nanogels seem to disappear in classical imaging modes since a sharp tip fully penetrates these porous networks with very low forces in the range of steric interactions (ca. 100 pN). However, the detailed evaluation of Force Volume mode measurements allows us to resolve their overall shape and at the same time their internal structure in all three dimensions. The nanogels exhibit an extraordinary disk‐like and entirely homogeneous but extremely soft structure—even softer than polymer brushes. Moreover, the temperature‐sensitive nanogels can be switched on demand between the ultra‐soft and a very stiff state.

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“…Complex coacervates were recognized to resemble these membraneless organelles and serve as cell model systems 94 , 95 and were proposed as protocells for the origin of life. 96 , 97 Since then, many interesting studies showed coacervates dynamic assembly and disassembly upon different stimuli 98 , 99 and their assembly into more complex, multiphase systems. 98 , 100 , 101 However, using complex coacervates, a polymer-rich phase is generated, as well as a polymer-poor phase.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

“… 96 , 97 Since then, many interesting studies showed coacervates dynamic assembly and disassembly upon different stimuli 98 , 99 and their assembly into more complex, multiphase systems. 98 , 100 , 101 However, using complex coacervates, a polymer-rich phase is generated, as well as a polymer-poor phase. This is not an accurate representation of the cell.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Exploring New Horizons in Liquid Compartmentalization via Microfluidics

Keller¹,

Teora²,

Boujemaa³

et al. 2021

Biomacromolecules

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Spatial organization of cellular processes is crucial to efficiently regulate life’s essential reactions. Nature does this by compartmentalization, either using membranes, such as the cell and nuclear membrane, or by liquid-like droplets formed by aqueous liquid–liquid phase separation. Aqueous liquid–liquid phase separation can be divided in two different phenomena, associative and segregative phase separation, of which both are studied for their membraneless compartmentalization abilities. For centuries, segregative phase separation has been used for the extraction and purification of biomolecules. With the emergence of microfluidic techniques, further exciting possibilities were explored because of their ability to fine-tune phase separation within emulsions of various compositions and morphologies and achieve one of the simplest forms of compartmentalization. Lately, interest in aqueous liquid–liquid phase separation has been revived due to the discovery of membraneless phases within the cell. In this Perspective we focus on segregative aqueous phase separation, discuss the theory of this interesting phenomenon, and give an overview of the evolution of aqueous phase separation in microfluidics.

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Synthesis of Polyampholyte Janus‐like Microgels by Coacervation of Reactive Precursors in Precipitation Polymerization

Cited by 25 publications

References 56 publications

Dendrimer-decorated nanogels: Efficient nanocarriers for biodistribution in vivo and chemotherapy of ovarian carcinoma

Dendrimer-decorated nanogels: Efficient nanocarriers for biodistribution in vivo and chemotherapy of ovarian carcinoma

Stiffness Tomography of Ultra‐Soft Nanogels by Atomic Force Microscopy

Exploring New Horizons in Liquid Compartmentalization via Microfluidics

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