Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium <i>Seliberia carboxydohydrogena</i> Z-1062

Volova, Tatiana G.; Zhila, Natalia O.; Shishatskaya, Ekaterina I.

doi:10.1007/s10295-015-1659-9

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…Polyhydroxyalkanoates (PHAs), degradable polyesters of microbial origin, are promising "green" plastics, which are degraded by natural microflora to CO 2 and H 2 O, causing no harm to biota and the entire environment [11][12][13][14][15][16][17]. PHAs are synthesized by prokaryotes from various substrates, including waste products [18][19][20][21]. Development of PHAs was a notable event for biotechnology of degradable materials [6,[22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

et al. 2021

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The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92° and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80–56° and surface energy and roughness increased to 41–57 mN/m and 172–290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76–80°) and reduced roughness parameters (to 40–45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67–76°), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment.

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Section: Introductionmentioning

confidence: 99%

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

et al. 2021

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“…Growth and PHB production as a function of CO content in the gas phase were investigated for Pseudomonas carboxydohydrogena , and there was no inhibitory effect of increasing CO concentration in syngas (from 10 to 30% v/v). The increasing CO content had a positive influence on the biomass production, but did not influence the cellular PHB content (Volova et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Fed-Batch Cultivations of Rhodospirillum rubrum Under Multiple Nutrient-Limited Growth Conditions on Syngas as a Novel Option to Produce Poly(3-Hydroxybutyrate) (PHB)

Karmann

Panke

Zinn

2019

Front. Bioeng. Biotechnol.

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Syngas from gasified organic waste materials is a promising feedstock for the biotechnological synthesis of the bioplastic poly([ R ]-3-hydroxybutyrate) (PHB) with Rhodospirillum rubrum . In a first approach, growth studies were carried out with this strain in gas-tight serum vials. When syngas (40% CO, 40% H 2 , 10% CO 2 , and 10% N 2 v/v) was diluted with N 2 to 60%, a 4-fold higher biomass production was detected compared to samples grown on 100% syngas, thus indicating a growth inhibitory effect. The best performing syngas-mixture was then used for C-, C,N-, and C,P-limited fed-batch fermentations in a bioreactor with continuous syngas and acetate supply. It was found that C,P-limited PHB productivity was 5 times higher than for only C-limited growth and reached a maximal PHB content of 30% w/w. Surprisingly, growth and PHB production stopped when N, as a second nutrient, became growth-limiting. Finally, it was concluded that a minimal supply of 0.2 g CO g −1 biomass h −1 has to be guaranteed in order to cover the cellular maintenance energy.

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“…This is because carbon monoxide dehydrogenase and acetyl-CoA synthase are connected via a channel that CO travels through [86]. When grown aerobically on 30% CO and limited via sulfur, Seliberia carboxydohydrogena Z-1062 was able to accumulate polyhydroxybutyrate (PHB) up to 63% [87]. Some components of crude syngas can inhibit cell growth, but these can be resolved by using charcoal filters [88].…”

Section: Carboxydotrophy (Co)mentioning

confidence: 99%

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Fukala,

Kučera

2024

Molecules

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Polyhydroxyalkanoates (PHAs) are intracellular biopolymers that microorganisms use for energy and carbon storage. They are mechanically similar to petrochemical plastics when chemically extracted, but are completely biodegradable. While they have potential as a replacement for petrochemical plastics, their high production cost using traditional carbon sources remains a significant challenge. One potential solution is to modify heterotrophic PHA-producing strains to utilize alternative carbon sources. An alternative approach is to utilize methylotrophic or autotrophic strains. This article provides an overview of bacterial strains employed for PHA production, with a particular focus on those exhibiting the highest PHA content in dry cell mass. The strains are organized according to their carbon source utilization, encompassing autotrophy (utilizing CO2, CO) and methylotrophy (utilizing reduced single-carbon substrates) to heterotrophy (utilizing more traditional and alternative substrates).

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Synthesis of poly(3-hydroxybutyrate) by the autotrophic CO-oxidizing bacterium Seliberia carboxydohydrogena Z-1062

Cited by 8 publications

References 24 publications

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition

Fed-Batch Cultivations of Rhodospirillum rubrum Under Multiple Nutrient-Limited Growth Conditions on Syngas as a Novel Option to Produce Poly(3-Hydroxybutyrate) (PHB)

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

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