Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration

Farooq, Ameeq; Yar, Muhammad; Khan, Abdul Samad; Shahzadi, Lubna; Siddiqi, Saadat Anwar; Mahmood, Nasir; Rauf, Abdur; Qureshi, Zafar-ul-Ahsan; Manzoor, Faisal; Chaudhry, Aqif Anwar; Rehman, Ihtesham Ur

doi:10.1016/j.msec.2015.06.006

Cited by 54 publications

(33 citation statements)

References 59 publications

(68 reference statements)

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“…Chitosan (CS) was purchased from Mian Scientific Company, Lahore, Pakistan and further purified in our laboratories as previously described 32,33 (degree of deacetylation (DD) 84%;…”

Section: Methodsmentioning

confidence: 99%

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

et al. 2016

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ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractThere is a need to develop pro-angiogenic biomaterials to promote wound healing and to assist in regenerative medicine. To this end, various growth factors have been exploited which have the potential to promote angiogenesis. However these are generally expensive and labile which limits their effectiveness. An alternative approach is to immobilize heparin onto biocompatible degradable hydrogels. The heparin in turn will then bind endogenous proangiogenic growth factors to induce formation of new blood vessels.In this study we continue our development of hydrogels for wound healing purposes by exploring covalently cross-linking chitosan (CS) and polyvinyl alcohol (PVA) hydrogels using triethyl orthoformate (TEOF) .Two concentrations of TEOF ( 4 and 16 %) were compared for their effects on the structure of hydrogels -their swelling, pore size and rate of degradation and 2 for their ability to support the growth of cells and for their heparin binding capacity and their effects on angiogenesis in a chick chorioantoic membrane assay .Hydrogels formed with 4 or 16% both TEOF cross-linker were equally cyto-compatible.Hydrogels formed with 4% TEOF absorbed slightly more water than those made with 16%TEOF and broke down slightly faster than non-cross-linked hydrogels. When soaked in heparin the hydrogel formed with 16% TEOF showed more blood vessel formation in the CAM assay than that formed with 4% TEOF.

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“…Chitosan (CS) was purchased from Mian Scientific Company, Lahore, Pakistan and further purified in our laboratories as previously described 32,33 (degree of deacetylation (DD) 84%;…”

Section: Methodsmentioning

confidence: 99%

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

et al. 2016

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“…Interestingly, functionalization with anti-inflammatory compounds such as alpha-melanocyte-stimulating hormone (α-MSH) has already displayed amplified anti-inflammatory effects associated with anti-soft tissue invasion and anti-fibrotic characteristics [ 28 ]. Fascinated by this concept, NSAID-loaded electrospun membranes (ketoprofen/PCL and piroxicam/chitosan) have also been successfully tested for periodontal regeneration in vitro with promising results [ 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study

et al. 2018

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Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aim of this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-ε-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalized membrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin α3β1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.

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“…In general, drug release from an electrospun polymer depends on many factors, including the drug solubility in the polymer solution, and the diffusion and biodegradability of the polymer matrix. [21][22][23] Drugpolymer compatibility, the drug solubility in the drug/ polymer/solvent system, can correlate with the ability to fully encapsulate drugs and accomplish sustained release. 9 The solvent evaporated quickly when the electrospinning solution was elongated rapidly, thus phase separation was avoided and the lipophilic drug was likely to remain inside the electrospun fibers.…”

Section: Discussionmentioning

confidence: 99%

A simvastatin-releasing scaffold with periodontal ligament stem cell sheets for periodontal regeneration

Zhao

Chen

Zhao

et al. 2020

Journal of Applied Biomaterials & Functional Materials

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Simvastatin (SIM) has been documented to induce the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). To establish an efficient release system for periodontal regeneration, a polycaprolactone (PCL) membrane scaffold containing SIM was electrospun and evaluated. The obtained PCL–SIM membrane scaffold showed sustained release up to 28 days, without deleterious effect on proliferation of PDLSCs on the scaffolds. PDLSCs were seeded onto scaffolds and their osteogenic differentiation was evaluated. After 21 days, expressions of collagen type I, alkaline phosphatase and bone sialoprotein genes were significantly upregulated and mineralized matrix formation was increased on the PCL–SIM scaffolds compared with the PCL scaffolds. In a heterotopic periodontal regeneration model, a cell sheet–scaffold construct was assembled by placement of multilayers of PDLSC sheets on PCL or PCL–SIM scaffolds, and these were then placed between dentin and ceramic bovine bone for subcutaneous implantation in athymic mice. After 8 weeks, the PCL–SIM membrane showed formation of significantly more ectopic cementum-like mineral on the dentin surface. These findings demonstrated that the PCL–SIM membrane scaffold promotes cementum-like tissue formation by sustained drug release, suggesting the feasibility of its therapeutic use with PDLSC sheets to improve periodontal regeneration.

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Synthesis of piroxicam loaded novel electrospun biodegradable nanocomposite scaffolds for periodontal regeneration

Cited by 54 publications

References 59 publications

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

Triethyl orthoformate covalently cross-linked chitosan-(poly vinyl) alcohol based biodegradable scaffolds with heparin-binding ability for promoting neovascularisation

Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study

A simvastatin-releasing scaffold with periodontal ligament stem cell sheets for periodontal regeneration

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