Synthesis of Pentapeptide FWKVV (Phe-Trp-Lys-Val-Val) and Its Activity as Antioxidants

Abstract

Antioxidant pentapeptides are pentapeptide compounds that have antioxidant activity. One of the pentapeptide compounds that have antioxidant activity is FWKVV. FWKVV is a linear pentapeptide with the amino acid sequence phenylalanine-tryptophan-lysine-valine-valine, which was first isolated to hydrolyzate the muscle protein of Miiuy croaker (Miichthysmiiuy). In addition to isolation, FWKVV compounds can be produced by the peptide synthesis method because this method requires a shorter time than the isolation method from natural materials. Synthesis methods commonly used are solution-phase peptide synthesis and solid-phase peptide synthesis (SPPS). However, the SPSS method is more efficient because it does not require purification in every process. The purpose of this study was to synthesize FWKVV compounds using the SPPS method and test their antioxidant activity. FWKVV has been synthesized using the SPPS method with HBTU/HOBt coupling reagent and Fmoc protective group. The FWKVV crud produced was 148.8 mg and had antioxidant activity against DPPH radicals with an IC50 value of 4.2 mg/mL.

Extraction of The Chemical Components of Dengen Leaves (Dillenia serrata Thunb) by MAE Method and Activity Test as Antioxidant and Toxicity

“…DPPH solution (blank) (1 mL), test solution (20, 40, 60, 80, 100 g/mL) (4 mL) + DPPH 0.3 mM (1 mL), and ascorbic acid solution (20, 40 , 60, 80, 100 g/mL) (4 mL) + DPPH 0.3 mM (1 mL) were incubated at 37 o C for 30 minutes. The absorbance of each solution was measured by UV-Vis spectrophotometer at a wavelength of 510-520 nm (Marliani et al, 2015;Dali et al, 2013;Dali et al, 2017;Souhoka et al, 2019;Yanti et al, 2021). The percentage of inhibition was calculated using Equation (1).…”

“…Dengen leaves ethanol extract solution (2 mL) was added with hot aquabidest (1 mL) and concentrated HCl (3 drops) and then shaken for 30 seconds. The saponin test was declared positive if a stable foam was formed for 1 minute (Jaafar et al, 2007;Yanti et al, 2021;Fadeyi et al, 2022).…”