Synthesis of oligonucleotide‐functionalized magnetic nanoparticles and study on their <i>in vitro</i> cell uptake

Wagner, Kerstin; Kautz, Armin R.; Röder, Michael; Schwalbe, Manuela; Pachmann, Katharina; Clement, Joachim H.; Schnabelrauch, Matthias

doi:10.1002/aoc.752

Cited by 64 publications

(48 citation statements)

References 14 publications

(10 reference statements)

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“…Carboxymethyl dextran-coated magnetic nanoparticles were prepared as previously described (Wagner et al 2004). Fluorescein-labeled carboxymethyl dextrancoated magnetic nanoparticles (FA-CM-dextran nanoparticles) were synthesized by thoroughly mixing 1 ml of a CM dextran nanoparticle dispersion (43.2 mg of CMdextran, degree of carboxymethyl groups = 1.5) at 0°C with a solution of N-(3-dimethylaminopropyl)-N¢-ethylcarbodiimide hydrochloride (EDC, 32.8 mg, 0.171 mmol) in 200 ll of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Clement

Schwalbe

Buske³

et al. 2006

J Cancer Res Clin Oncol

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Section: Methodsmentioning

confidence: 99%

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Clement

Schwalbe

Buske³

et al. 2006

J Cancer Res Clin Oncol

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“…The nanoparticles were internalized depending on tumor cell size and morphology, which led to widespread heterogeneity. [42][43][44][45] For instance, some cancer cells solely internalized during division. 46 As opposed to dextran, Resovist is coated with carboxydextran and is a liver-specific magnetic resonance contrast agent.…”

mentioning

confidence: 99%

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Lindemann¹,

Lüdtke‐Buzug²,

Fräderich³

et al. 2014

IJN

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Background As a tomographic imaging technology, magnetic particle imaging (MPI) allows high spatial resolution and sensitivity, and the possibility to create real-time images by determining the spatial distribution of magnetic particles. To ensure a prospective biosafe application of UL-D (University of Luebeck-Dextran coated superparamagnetic nanoparticles), we evaluated the biocompatibility of superparamagnetic iron oxide nanoparticles (SPIONs), their impact on biological properties, and their cellular uptake using head and neck squamous cancer cells (HNSCCs). Methods SPIONs that met specific MPI requirements were synthesized as tracers. Labeling and uptake efficiency were analyzed by hematoxylin and eosin staining and magnetic particle spectrometry. Flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, and real-time cell analyzer assays were used to investigate apoptosis, proliferation, and the cytokine response of SPION-labeled cells. The production of reactive oxygen species (ROS) was determined using a fluorescent dye. Experimental results were compared to the contrast agent Resovist ® , a standard agent used in MPI. Results UL-D nanoparticles and Resovist particles were taken up in vitro by HNSCCs via unspecific phagocytosis followed by cytosolic accumulation. To evaluate toxicity, flow cytometry analysis was performed; results showed that dose- and time-dependent administration of Resovist induced apoptosis whereas cell viability of UL-D-labeled cells was not altered. We observed decreased cell proliferation in response to increased SPION concentrations. An intracellular production of ROS could not be detected, suggesting that the particles did not cause oxidative stress. Tumor necrosis factor alpha (TNF-α) and interleukins IL-6, IL-8, and IL-1β were measured to distinguish inflammatory responses. Only the primary tumor cell line labeled with >0.5 mM Resovist showed a significant increase in IL-1β secretion. Conclusion Our data suggest that UL-D SPIONs are a promising tracer material for use in innovative tumor cell analysis in MPI.

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“…Carboxymethyl dextran sodium salt (CMD, degree of substitution (DS) = 0.8) was prepared by etherification of dextran (Fluka, Buchs, Switzerland, from Leuconostoc spp., M = 15,000 -20,000 g/mol) with monochloroacetic acid in an isopropanol/NaOH mixture according to a known procedure [29].…”

Section: Methodsmentioning

confidence: 99%

Dextran‐based coating system for the immobilization of cell adhesion promoting molecules on titanium surfaces

Dubs

Weisser

Linke

et al. 2009

Materialwissenschaft Werkst

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Immobilization of adhesive peptides interacting with cellular integrin receptors onto metallic implant surfaces represents a promising approach to improve osseointegration of implants into the surrounding tissue. In the present study, a functional dextran-based coating system consisting of an amino titanate adhesion promoter with dendritic structure and a carboxymethyl dextran was established to bind an RGD-containing adhesive peptide via a selective coupling methodology onto titanium surfaces. The three-step reaction procedure was characterized by X-ray photoelectron spectroscopy. In cell adhesion experiments it could be demonstrated that dextran coatings containing immobilized RGD promote attachment and spreading of fibroblast and pre-osteoblastic cells compared to native as well as CMD-coated titanium surfaces without RGD. The direct attachment of the RGD sequence to the metal surface via the amino titanate adhesion promoter did not increase pre-osteoblastic cell spreading, whereas coupling of RGD to the polymeric carboxymethyl dextran layer slightly enhanced spreading of the cells.

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Synthesis of oligonucleotide‐functionalized magnetic nanoparticles and study on their in vitro cell uptake

Cited by 64 publications

References 14 publications

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Dextran‐based coating system for the immobilization of cell adhesion promoting molecules on titanium surfaces

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