Synthesis of oligo(ethylene glycol) methacrylate polymer brushes

Brown, Andrew A.; Khan, Neelam; Steinbock, Lorenz J.; Huck, Wilhelm T. S.

doi:10.1016/j.eurpolymj.2005.02.026

Cited by 124 publications

(149 citation statements)

References 27 publications

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“…The glass surface is initially functionalized with a commercially available, amine-terminated silane, which is easily modified to present the bromoisobutyrate initiator. [3,50] ATRP in general, and that of OEGMA in particular, has several important attributes that are relevant for the modification of surfaces of diverse materials with a protein-resistant coating. First, ATRP initiators can be easily immobilized onto surfaces by various methods, such as covalent coupling, Langmuir-Blodgett deposition, plasma grafting, and chemical self-assembly.…”

Section: Growth Of Poegma-functionalized Polymer Brushes On Surfacesmentioning

confidence: 99%

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

2009

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Protein resistant or “non‐fouling” surfaces are of great interest for a variety of biomedical and biotechnology applications. This article briefly reviews the development of protein resistant surfaces, followed by recent research on a new methodology to fabricate non‐fouling surfaces by surface‐initiated polymerization. We show that polymer brushes synthesized by surface‐initiated polymerization that present short oligo(ethylene glycol) side chains are exceptionally resistant to protein adsorption and cell adhesion. The importance of the protein and cell resistance conferred by these polymer brushes is illustrated by their use as substrates for the fabrication of antibody microarrays that exhibit femtomolar limits of detection in complex fluids such as serum and blood with relaxed requirements for intermediate wash steps. This example highlights the important point that the reduction in background noise afforded by protein‐resistant surfaces can greatly simplify the development of ultrasensitive heterogeneous, surface‐based clinical and proteomic assays with increased sensitivity and utility.

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Section: Growth Of Poegma-functionalized Polymer Brushes On Surfacesmentioning

confidence: 99%

In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins

2009

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“…It is well known that organic polymer coatings permit to tune the surface properties of materials such as wetting, [1] adhesion, [2] chemical sensing; [3,4] response to chemical, temperature, pH, ionic strength, light, and mechanical stimuli; [5] cell adhesion, [6] resistance to protein adsorption [7] and bacterial fouling, [8] to name but a few.…”

Section: Introductionmentioning

confidence: 99%

Controlled adhesion of Salmonella Typhimurium to poly(oligoethylene glycol methacrylate) grafts

Mrabet

Mejbri

Mahouche

et al. 2011

Surface & Interface Analysis

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International audiencePoly(oligoethylene glycol methacrylate), POEGMA, brushes were prepared by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated silicon wafers. Prior to ATRP, the substrates were grafted by brominated aryl initiators via the electrochemical reduction of a noncommercial parent diazonium salt of the formula BF4-, N-+(2)-C6H4-CH(CH3)Br. The diazonium-modified gold plates (Au-Br) served as macroinitiators for ATRP of OEGMA which resulted in hydrophilic surfaces (Au-POEGMA) that could be used for two distinct objectives: (i) resistance to fouling by Salmonella Typhimurium; (ii) specific recognition of the same bacteria provided that the POEGMA grafts are activated by anti-Salmonella. The Au-POEGMA plates were characterized by XPS, polarization modulation-infrared reflection-absorption spectroscopy (PM-IRRAS) and contact angle measurements. Both Beer-Lambert equation and Tougaard's QUASES software indicated a POEGMA thickness that exceeds the critical similar to 10 nm value necessary for obtaining a hydrophilic polymer with effective resistance to cell adhesion. The Au-POEGMA slides were further activated by trichlorotriazine (TCT) in order to covalently bind anti-Salmonella antibodies (AS). The antibody-modified Au-POEGMA specimens were found to specifically attach Salmonella Typhimurium bacteria. This work is another example of the diazonium salt/ATRP process to provide biomedical polymer surfaces

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“…[18][19][20] The procedure used to grow the POEGMA brushes on glass is summarized in Figure 1. We have previously shown that a thickness of POEGMA > 10 nm on gold and silicon oxide resists nonspecific protein adsorption.…”

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confidence: 99%