Synthesis of novel Fe3O4@SiO2@CeO2 microspheres with mesoporous shell for phosphopeptide capturing and labeling

Cheng, Gong; Zhang, Jilin; Liu, Yanlin; D, Sun; Ni, Jiazuan

doi:10.1039/c1cc10533g

Cited by 117 publications

(83 citation statements)

References 25 publications

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“…Milk has been widely used as a real complex sample for phosphopeptide enrichment in previous literature. 37,38 In our experiment, the protein concentration of this drinking milk is about 3%, indicated by the milk producer. Figure 8a shows a mass spectrum obtained from direct analysis of 1 μL of tryptic digests of drinking milk; only three weak phosphopeptide peaks appeared before enrichment.…”

Section: Enrichment Of Phosphopeptides Derived From Standard Proteinsmentioning

confidence: 98%

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Zhang

et al. 2013

ACS Appl. Mater. Interfaces

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Designed with a two-in-one strategy, the magnetic mesoporous γ-Fe 2 O 3 nanocrystal clusters (m-γ-Fe 2 O 3 ) have been successfully prepared for integrating the functions of effective enrichment and quick separation of phosphopeptides into a single architecture. First, the mesoporous Fe 3 O 4 nanocrystal clusters (mFe 3 O 4 ) were synthesized by solvothermal reaction and then were subjected to calcination in air to form m-γ-Fe 2 O 3 . The obtained m-γ-Fe 2 O 3 have spherical morphology with uniform particle size of about 200 nm and mesoporous structure with the pore diameter of about 9.7 nm; the surface area is as large as 117.8 m 2 /g, and the pore volume is 0.34 cm 3 /g. The m-γ-Fe 2 O 3 possessed very high magnetic responsiveness (Ms = 78.8 emu/g, magnetic separation time from solution is less than 5 s) and were used for the selective enrichment of phosphopeptides for the first time. The experimental results demonstrated that the m-γ-Fe 2 O 3 possessed high selectivity for phosphopeptides at a low molar ratio of phosphopeptides/nonphosphopeptides (1:100), high sensitivity (the detection limit was at the fmol level), high enrichment recovery (as high as 89.4%), and excellent speed (the enrichment can be completed in 10 min). Moreover, this material is also quite effective for enrichment of phosphopeptides from the real sample (drinking milk), showing great potential in the practical application.

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Section: Enrichment Of Phosphopeptides Derived From Standard Proteinsmentioning

confidence: 98%

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Zhang

et al. 2013

ACS Appl. Mater. Interfaces

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“…It has been demonstrated that a group of metal oxides, such as TiO 2 , ZrO 2 , Fe 2 O 3 , and Al 2 O 3 , are effective in enriching phosphorylated proteins by means of electrostatically driven binding or coordinate covalent interactions (Leitner et al 2011;Li et al 2008;Lu et al 2010b). The nano-scaled morphology (for example, mesoporous or not) of the metal oxides is an important factor in achieving high efficiency in the enrichment of the target proteins (Lu et al 2010a;Cheng et al 2011;Ma et al 2012). On the other hand, the enrichment performance of those materials is also heavily impacted by the formats of their usage.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of BC@mTiO2 hybrid nanofibers for highly efficient enrichment and detection of phosphopeptides

Gao

Chen

et al. 2016

Cellulose

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Along with advances in life science and clinical research, there has been an increasing interest in enrichment technologies for proteins with posttranslational modifications. Here we report a new platform to enrich and detect phosphopeptides using the hybrid nanofibers synthesized from bacterial cellulose (BC). Hydrothermal reactions have successfully been employed to synthesize BC@mTiO 2 hybrid nanofibers. The morphology of the hybrid nanofibers has been characterized in detail. They are featured with tremendously increased specific surface areas and appropriate pore size for adsorption of phosphopeptides with high efficiency. The BC@mTiO 2 tips allow improving both the sensitivity and selectivity of mass spectrometry by nearly two orders of magnitude compared with the commercial tips. As a robust and highly cost-effective platform, our approach has provided a nanotechnology invention to enrich and detect phosphorylated proteins with important biomedical applications.

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“…CeO 2 ). [7] Finally, to enhance relevant MS signals by increasing the adsorptive capacity and selectivity, some mesoporous oxides [8] and metal ions immobilized on mesoporous silica particles [9] have been developed for the selective adsorption and separation of phosphopeptides, owing to their large specific surface area and in-pore surface area with a higher number of affinity sites. Although the MS signals can be observably improved, the "shadow effect" from the smaller and deeper pores is often unavoidable and hampers the release of target biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

Cheng

Zhang

Liu

et al. 2012

Chemistry A European J

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Rare-earth phosphate microspheres with unique structures were developed as affinity probes for the selective capture and tagging of phosphopeptides. Prickly REPO(4) (RE = Yb, Gd, Y) monodisperse microspheres, that have hollow structures, low densities, high specific surface areas, and large adsorptive capacities were prepared by an ion-exchange method. The elemental compositions and crystal structures of these affinity probes were confirmed by energy-dispersive spectroscopy (EDS), powder X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy. The morphologies of these compounds were investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen-adsorption isotherms. The potential ability of these microspheres for selectively capturing and labeling target biological molecules was evaluated by using protein-digestion analysis and a real sample as well as by comparison with the widely used TiO(2) affinity microspheres. These results show that these porous rare-earth phosphate microspheres are highly promising probes for the rapid purification and recognition of phosphopeptides.

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Synthesis of novel Fe3O4@SiO2@CeO2 microspheres with mesoporous shell for phosphopeptide capturing and labeling

Abstract: Fe(3)O(4)@SiO(2)@CeO(2) microspheres with magnetic core and mesoporous shell were synthesized, and the multifunctional materials were utilized to capture phosphopeptides and catalyze the dephosphorylation simultaneously, thereby labeling the phosphopeptides for rapid identification.

Cited by 117 publications

References 25 publications

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Synthesis of BC@mTiO2 hybrid nanofibers for highly efficient enrichment and detection of phosphopeptides

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

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Synthesis of novel Fe3O4@SiO2@CeO2 microspheres with mesoporous shell for phosphopeptide capturing and labeling

Abstract: Fe(3)O(4)@SiO(2)@CeO(2) microspheres with magnetic core and mesoporous shell were synthesized, and the multifunctional materials were utilized to capture phosphopeptides and catalyze the dephosphorylation simultaneously, thereby labeling the phosphopeptides for rapid identification.

Cited by 117 publications

References 25 publications

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe2O3 Nanocrystal Clusters

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe2O3 Nanocrystal Clusters

Synthesis of BC@mTiO2 hybrid nanofibers for highly efficient enrichment and detection of phosphopeptides

Monodisperse REPO4 (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides

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Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous γ-Fe₂O₃ Nanocrystal Clusters

Monodisperse REPO₄ (RE=Yb, Gd, Y) Hollow Microspheres Covered with Nanothorns as Affinity Probes for Selectively Capturing and Labeling Phosphopeptides