Synthesis of new ultrasonic-assisted palladium oxide nanoparticles: an in vitro evaluation on cytotoxicity and DNA/BSA binding properties

Sorinezami, Ziba; Mansouri‐Torshizi, Hassan; Aminzadeh, Mohammad; Ghahghaei, Arezou; Jamgohari, Nasimeh; Majd, Mostafa Heidari

doi:10.1080/07391102.2018.1546619

Cited by 22 publications

(11 citation statements)

References 63 publications

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“…49 Cell viability decreased with the increased concentration, which implies that our synthesized compound may be active against lung cancer which is beneficial for future drug-design perspectives. 50…”

Section: Resultsmentioning

confidence: 99%

Effect of Reaction Temperature on Shape Evolution of Palladium Nanoparticles and Their Cytotoxicity against A-549 Lung Cancer Cells

Abbas

Kumar

et al. 2019

ACS Omega

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Palladium nanoparticles (Pd NPs) of different shapes and sizes have been synthesized by reducing potassium tetrachloropalladinate(II) by l-ascorbic acid (AA) in an aqueous solution phase in the presence of an amphiphilic nonionic surfactant poly ethylene glycol (PEG) via a sonochemical method. Materials have been characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray soectrscopy (EDX), Fourier transform infrared (FTIR), surface-enhanced Raman spectroscopy (SERS), particle distribution, and zeta potential studies. Truncated octahedron/fivefold twinned pentagonal rods are formed at room temperature (RT) (25 °C) while hexagonal/trigonal plates are formed at 65 °C. XRD results show evolution of anisotropically grown, phase-pure, and well crystalline face-centered cubic Pd NPs at both temperatures. FTIR and SERS studies revealed adsorption of ascorbic acid (AA) and PEG at NP’s surface. Particle’s size distribution graph indicates formation of particles having wide size distribution while the zeta potential particle surface is negatively charged and stable. The truncated octahedron/fivefold twinned pentagonal rod-shaped Pd NPs, formed at RT, while thermally stable and kinetically controlled hexagonal/trigonal plate-like Pd NPs, evolved at higher temperature 65 °C. The obtained Pd NPs have a high surface area and narrow pore size distribution. To predict protein reactivity of the Pd cluster, docking has been done with DNA and lung cancer-effective proteins. The cytotoxicity of the Pd NPs has been screened on human lung cancer cells A-549 at 37 °C. The biological adaptability exhibited by Pd NPs has opened a pathway in biochemical applications.

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Section: Resultsmentioning

confidence: 99%

Effect of Reaction Temperature on Shape Evolution of Palladium Nanoparticles and Their Cytotoxicity against A-549 Lung Cancer Cells

Abbas

Kumar

et al. 2019

ACS Omega

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“…Therefore, the value of K q is obtained as 1.2 × 10 11 M −1 s −1 which is greater than the maximum diffusion collision rate (2.0 × 10 10 M −1 s −1 ) for a biological molecule. This indicates that the Static quenching mechanism is involved in the interaction of BSA with Se‐Met‐Fa NPs 18 …”

Section: Resultsmentioning

confidence: 85%

“…Hence, the literature suggests no prominent peak of selenium in the infrared region. 18,19 In the current study, the FTIR technique helped to study the peak intensity, vibrations, and shifting in the infrared region by any interactions of methionine and folic acid. Figure 1C shows 3415 cm À1…”

Section: Discussionmentioning

confidence: 99%

“…10 11 M À1 s À1 which is greater than the maximum diffusion collision rate (2.0 Â 10 10 M À1 s À1 ) for a biological molecule. This indicates that the Static quenching mechanism is involved in the interaction of BSA with Se-Met-Fa NPs 18. The far UV-CD spectra of BSA were monitored in the presence of Se NPs, Se-Met NPs, and Se-Met-Fa NPs to study the alteration in the secondary structure of BSA on interacting with all three nanoparticles.…”

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confidence: 99%

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In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

Shinde

Desai²

2022

J Biomed Mater Res

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The present research focuses on synthesizing selenium nanoparticles (Se NPs) coated with Methionine and Folic acid. selenium‐methionine‐folate nanoparticles (Se‐Met‐Fa NPs) were prepared by the chemical precipitation method. These were characterized by UV–Vis spectroscopy, FTIR, X‐ray diffraction, Zeta potential, ICP‐AES, TEM, and Raman spectroscopy. The average diameter of nanoparticles was determined by TEM was 50 nm. In Vitro viability of cells exposed to Se‐Met‐Fa NPs were studied using MTT and AO/EB assay. Approximately 80% of cells were viable at 100 μg/ml concentration after 24 h of incubation, suggestive of the safety of nanoparticles. Macromolecular interaction studies were carried out with plasmid DNA and Bovine serum albumin (BSA) protein. UV–Vis Spectroscopy showed ground state complex formation of Se‐Met‐Fa NPs with BSA. Intrinsic fluorescence of BSA was quenched by Se‐met‐Fa NPs via Static quenching and was observed under Spectro‐fluorimetry. Conformational structural change in α‐Helices of BSA was observed to be 4.4% after the interaction with Se‐Met‐Fa NPs, and it was studied using CD spectroscopy. At 250 μg/ml Se‐Met‐Fa NPs prevented oxidative damage of Plasmid DNA. The total antioxidant property of Se‐Met‐Fa NPs expressed in terms of scavenging of free DPPH radicals. Ten micrograms per milliliter could inhibit 41% of DPPH, proving its scavenger role at the lowest concentration. Nanoparticles comprising antioxidant semi‐conducting cores and encapsulated by biomaterials that are highly bioavailable can be promising therapeutic agents for inflammation and oxidative stress disorders studies.

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“…According to existing guidelines, human lung cancer cell lines (A549) and human cervical cancer HeLa cell lines were grown for 1 week in monolayer 25‐cm 2 culture flasks. [ 13–15 ] The cells were then moved into 96‐well culture plates at a density of 1.2 × 10 4 cells per well and incubated for 24 h in a 5% CO 2 incubator. In the following, the cells were treated with five different doses (from 50 to 800 μM) of both [Zn(Phen)FA] and cisplatin for 48 and 72 h under aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

The in vitro apoptotic effect of new zinc complex possessing folic acid and phenanthroline on cervix cancer cells

Yang

Majd

Shiri

et al. 2021

Applied Organom Chemis

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With proper care and nursing as well as timely diagnosis and good treatment, cervical cancer is one of the most treatable forms of cancer. One of the most important cancer treatments is the use of emerging developing metal–organic complexes, which can provide a wide variety of morphologies, sizes, and properties based on different metal ions and different organic ligands. Accordingly, we reported the synthesis method, structural properties, cytotoxicity, apoptotic properties, and molecular docking analysis of the new Zn(II) complex consisting of phenanthroline and folic acid ligands. FTIR, 1H‐NMR, 13C‐NMR, and elemental analyzer determined that the synthesis of the complex was successful. The IC50 values (380 and 199 μM) were shown that the viability of HeLa cells was dependent on time of exposure and concentration of [Zn(Phen)FA] complex. Real‐time PCR clearly shows increased expression of apoptotic genes in cervical carcinoma HeLa cells and a possible relationship between Bak1/Bclx ratio and caspase‐3. Also, the key amino acids of folate receptor that can interact with the synthesized complex were identified by molecular docking analysis. Moreover, molecular electrostatic potential confirmed that carboxylate portion of FA is an electron‐rich region and can interact with Zn(Phen) to form a stable and soluble complex. All experiments confirmed that presence of FA could improve stability, cytotoxicity, apoptosis, and cellular uptake of complex in cervical carcinoma HeLa cells.

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Synthesis of new ultrasonic-assisted palladium oxide nanoparticles: an in vitro evaluation on cytotoxicity and DNA/BSA binding properties

Cited by 22 publications

References 63 publications

Effect of Reaction Temperature on Shape Evolution of Palladium Nanoparticles and Their Cytotoxicity against A-549 Lung Cancer Cells

Effect of Reaction Temperature on Shape Evolution of Palladium Nanoparticles and Their Cytotoxicity against A-549 Lung Cancer Cells

In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

The in vitro apoptotic effect of new zinc complex possessing folic acid and phenanthroline on cervix cancer cells

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