Synthesis of Nanophase Iron Oxide in Lumazine Synthase Capsids

Shenton, Wayne; Mann, Stephen; Cölfen, Helmut; Bacher, Adelbert; Fischer, Markus

doi:10.1002/1521-3757(20010119)113:2<456::aid-ange456>3.0.co;2-7

Cited by 25 publications

(10 citation statements)

References 17 publications

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“…This anomalous kinetic behavior has been attributed to substrate channeling due to the confinement of the RF synthase module inside the icosahedral capsids (114,224). It is noteworthy that the cavity of recombinant icosahedral lumazine synthase capsids can be used in vitro for the containment of nanocrystalline iron oxide (428). The 3-dimensional structures of RF synthases from E. coli, S. pombe, and plants have been determined by X-ray crystallography, and their intramolecular sequence structures are very similar.…”

Section: Riboflavin Synthasementioning

confidence: 95%

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Abbas

Sibirny

2011

Microbiol Mol Biol Rev

316

272

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SUMMARY Riboflavin [7,8-dimethyl-10-(1′- d -ribityl)isoalloxazine, vitamin B 2 ] is an obligatory component of human and animal diets, as it serves as the precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which are involved in oxidative metabolism and other processes. Commercially produced riboflavin is used in agriculture, medicine, and the food industry. Riboflavin synthesis starts from GTP and ribulose-5-phosphate and proceeds through pyrimidine and pteridine intermediates. Flavin nucleotides are synthesized in two consecutive reactions from riboflavin. Some microorganisms and all animal cells are capable of riboflavin uptake, whereas many microorganisms have distinct systems for riboflavin excretion to the medium. Regulation of riboflavin synthesis in bacteria occurs by repression at the transcriptional level by flavin mononucleotide, which binds to nascent noncoding mRNA and blocks further transcription (named the riboswitch). In flavinogenic molds, riboflavin overproduction starts at the stationary phase and is accompanied by derepression of enzymes involved in riboflavin synthesis, sporulation, and mycelial lysis. In flavinogenic yeasts, transcriptional repression of riboflavin synthesis is exerted by iron ions and not by flavins. The putative transcription factor encoded by SEF1 is somehow involved in this regulation. Most commercial riboflavin is currently produced or was produced earlier by microbial synthesis using special selected strains of Bacillus subtilis , Ashbya gossypii , and Candida famata . Whereas earlier RF overproducers were isolated by classical selection, current producers of riboflavin and flavin nucleotides have been developed using modern approaches of metabolic engineering that involve overexpression of structural and regulatory genes of the RF biosynthetic pathway as well as genes involved in the overproduction of the purine precursor of riboflavin, GTP.

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Section: Riboflavin Synthasementioning

confidence: 95%

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Abbas

Sibirny

2011

Microbiol Mol Biol Rev

316

272

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“…此时, 通过离子扩散速率和微环境的调节, 如使用凝胶或多孔介质控制晶体生长速度, 或人工构建如蛋白质笼和空心酶、脂质体 [117,118] 、微胶、微乳等类似于磁小体囊膜的结构 [106,119,120] , 以囊泡体积大小限制晶体的形状及大小. 此外, 不同金属成分的添加及条件控制, 可以对纳米晶体的组分进行调节 [27] .…”

Section: 磁小体的体外仿生unclassified

Biosynthesis and application of magnetosomes in tumor-targeting therapy

Zhong¹,

Li²,

Fu³

et al. 2017

Sci. Sin.-Vitae

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“…Indeed, a thin and continuous coating of the inner wall or complete filling of the ferritin cavity was successively demonstrated recently for cobalt oxide [39]. A similar approach has been applied to other proteins with spherical cage morphology such as lumazine synthase [40], the ferritin-like protein from Listeria innocua [41], or the heat shock protein from Methanococcus jannaschii [42,43]. The native protein shells are usually suitable for the growth of only a limited number of materials, so that chemical modification of the peptide subunits is required to expand the potential of this approach to a wider range of inorganic materials.…”

Section: Proteinsmentioning

confidence: 99%

Synthesis and Assembly of Nanoparticles and Nanostructures Using Bio‐Derived Templates

Dujardin

Mann

2007

Nanobiotechnology II

Self Cite

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The emergence of complexity is a defining feature of our planet. Moreover, complexity is ubiquitous. It is observed in inanimate and animate matter, social systems and communication protocols, and in diverse environmental networks. In each case, these systems exhibit at a fundamental level organizational properties that emerge without the intervention of any distinct organizing agent. This notion provides a formidable challenge for scientists attempting to decipher the general and specific processes responsible for complexity, and is a source of deep inspiration for those who attempt to shape and organize matter through synthetic procedures [1]. Over the past few decades, it has been realized that morphological, structural and functional complexity is often a defining characteristic of biomineralization, which generally involves temporally and spatially dependent interactions between assembling organic scaffolds and inorganic minerals [2]. Fortunately, the overwhelming variety found in biomineralization (and Nature in general) does not necessarily arise from a cumulative complexity involving excessive complication and layering of increasing numbers of components and networks, but rather from an elegant complexity where a limited number of principles and building blocks are combined in a multitude of ways to produce adaptive and evolutive structures with precise functions. This second paradigm offers hope that complexity can also be achieved in synthetic materials if the key concepts and processes can be distilled from the appropriate biological archetypes. For complex nanostructures, a good starting point is to develop experimental approaches based on principles of biomineralization, such as templated nucleation, growth and directed self-assembly. Thus, in this chapter we illustrate how unraveling the specific interactions between bio-derived templates and inorganic materials not only yields a better understanding of natural hybrid materials but also inspires new methods for developing the potential of biological molecules, superstructures and organisms as self-assembling agents for materials fabrication. 39 Nanobiotechnology II. Edited by Chad A. Mirkin and Christof M. Niemeyer

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Synthesis of Nanophase Iron Oxide in Lumazine Synthase Capsids

Cited by 25 publications

References 17 publications

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Biosynthesis and application of magnetosomes in tumor-targeting therapy

Synthesis and Assembly of Nanoparticles and Nanostructures Using Bio‐Derived Templates

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