The synthesis of nano-CaO can be produced from natural resources, chemical compounds, and waste containing calcium such as clamshell (Geloine sp.). The research aims to study the effect of temperature and reaction time (sol formation) on the yield of nano-CaO after the calcination process and to characterize the Ca(OH) 2 and nano-CaO obtained. The result shows that clamshell (Geloine sp.) waste can be used as raw material for the synthesis of nano-CaO using the sol-gel method. The main factor in the synthesis of nano-CaO by sol-gel method is sol formation from the calcium precursor with hydrochloric acid to form calcium chloride. The optimum temperature of sol formation is at the temperature of 90 -100°C for 60 minutes of reaction time with a yield of nano-CaO of 11.34 -12%. The optimum reaction time of gel formation is the reaction time of 90 minutes at a temperature of 100°C with a yield of nano-CaO of 14.5%. The yield of CaO decreases after the reaction time of 90 minutes. It is can be concluded that the optimum conditions for gel formation are a temperature of 100°C and a reaction time of 90 minutes. The nano-CaO can be used for any purpose, such as an additive to body soap because of its antimicrobial activity.