Synthesis of Multifunctional Polyvinylsaccharide Containing Controllable Amounts of Biospecific Ligands

Abstract

In the present study, the attempt to synthesize a multibiofunctional polymeric vector to be used for construction of composite scaffolds for bone tissue engineering has been undertaken. The polymers based on 2-deoxy-2-methacrylamido- d-glucose were functionalized by a growth factor (BMP-2), GRGDSP peptide, and poly( l-lysine) using aldehyde chemistry. The covalent modification process was quantitatively studied, and a polymer conjugate containing all these ligands was formed. In addition, the impacts of coupled ligands toward the adsorption of polymers on the commercial mineral macroporous matrix Sponceram used in cell culture applications were studied.

Monolithic bioreactors: Effect of chymotrypsin immobilization on its biocatalytic properties

“…Additionally, two macromolecular spacers, namely, copolymer of N-vinylpyrrolidone with acrolein diethyl acetal, containing 21 mol% of DAAc, MW 25,000, and terpolymer of 2-deoxy-N-methacryloylamido-d-glucose with N-vinylpyrrolidone and acrolein diethyl acetal containing 8 mol% of DAAc, MW 18,000, were selected for investigation. Both of chosen copolymers represent water-soluble and biocompatible macromolecules [46,47]. The introduction of saccharide residues (MAG) into the polymer structure can provoke the formation of enzyme microenvironment close to its natural medium.…”

A study on the influence of biocompatible composites with bioactive ligands toward their effect on cell adhesion and growth for the application in bone tissue engineering

“…After oxidation, the molar amount of aldehyde groups in the polymer averages 30%. 20 To achieve aldehyde groups in the polymer p(MVD) the diethylacetal groups of acrolein were transformed into aldehydes using hydrochloric acid ( Figure 3). Therefore, 50 mg p(MVD) was dissolved in 10 mL hydrochloric acid solution, pH 5 2.…”

“…19,20 These were used to couple the bioactive ligands covalently via their amino groups. The great advantage of this chemical procedure is its practicability under mild conditions and the prevention of toxic by-products.…”

“…19,20,25,26 The presence of saccharide groups in such polymers is suitable because saccharides play an essential biological role in vivo. Because of that, using saccharidebased material is an attempt to mimic ECM features and get a material comparably to natural ones.…”

“…The used spacers are biocompatible, but nondegradable polyvinylsaccharides, namely polymers and copolymers of 2-deoxy-N-methacrylamido-D-glucose, with adequate molecular weight and a controllable amount of aldehyde groups. 19,20 These were used to couple the bioactive ligands covalently via their amino groups. The great advantage of this chemical procedure is its practicability under mild conditions and the prevention of toxic by-products.…”