Synthesis of Membrane and Periplasmic Proteins during Starvation of a Marine Vibrio sp.

Nyström, Thomas; Albertson, Nan H.; Kjelleberg, Staffan

doi:10.1099/00221287-134-6-1645

Cited by 31 publications

(37 citation statements)

References 21 publications

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“…Previous studies reported that nutrient deprivation caused some physiological changes of marine bacterial cells in cell volume, endogenous metabolism protein composition and so on (Novitsky & Morita 1978, Geesey & Morita 1981, Kurath & Morita 1983, MBrden et al 1985, Nystrom et al 1988. A copiotrophic bactenum, S14, which possessed 2 leucine uptake systems of low and high affinities, acitvated the high-affinity system under starved conditions (Marden et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean

Yoshinaga¹,

Fukami²,

Ishida³

1991

Mar. Ecol. Prog. Ser.

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DNA and protein synthesis activities of Pacific bacterial assemblages in coral reefs of Ponape Island (eutrophic water), Majero Atoll (mesotrophic water) and pelagic waters (oligotrophic water) were estimated by measuring incorporation rates of 3H-thymidine and 3H-leucine into the TCAinsoluble fraction (TdRDNA, Leupro,). Bacterial production rates estimated by TdRDNA were 0.14 to 0.94 (average 0.52) X 104 cells ml-' h-' in the pelagic water and 0.93 to 26 (average 10) x 104 cells ml-' h-' in the eutrophic water Obligate oligotrophs predominated in the pelagic water, but facultative oligotrophs were dominant in the eutrophic water. In the eutrophic water protein synthesis rates expressed by Leu,,,, were positively correlated to DNA synthesis rates expressed by TdR DN*; however, in the oligotrophic pelagic water, there was no such correlation. Uptake activities of leucine at low concentration were maintained at relatively high levels in the oligotrophic water although 3~-t h y m i d i n e incorporation rates were extremely low. From these results, it is suggested that bacterial assemblages in oligotrophic waters have an uptake system with high affinity to substrates and utilize metabolic energy to achieve substrate uptake and protein synthesis preferentially to DNA synthesis and reproduction, and that these properties were mostly due to obligately oligotrophic bacteria adapted to a low nutrient environment.

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Section: Discussionmentioning

confidence: 99%

Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean

Yoshinaga¹,

Fukami²,

Ishida³

1991

Mar. Ecol. Prog. Ser.

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“…S14. The latter strain responds to starvation conditions by a complex reorganization of metabolic activities including synthesis and degradation of proteins (Nystrom et al, 1988, fatty acids (Malmcrona-Fri berg et al, 1986), DNA (Mirdkn et al, 1988), and cell wall peptidoglycan . These adjustments result in a developmental form which is highly adapted for survival and is resistant to multiple stress conditions including ampicillin-induced autolysis , sonic lysis (Nystrom & Kjelleberg, N …”

Section: Introductionmentioning

confidence: 99%

Macromolecular synthesis during recovery of the marine Vibrio sp. S14 from starvation

Albertson

Nyström

Kjelleberg

1990

Journal of General Microbiology

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The recovery of Vibrio sp. S14 cells from energy-and nutrient-starvation was monitored after the addition of glucose minimal medium. Upshift experiments were done throughout a starvation period of 200 h to determine whether cells were more responsive to nutrient addition at the onset of starvation, or if the previously described programme of starvation-induced cellular reorganization had to be completed before cells could become committed to recovery following nutritional upshifts. The kinetics of macromolecular synthesis (RNA, protein and DNA), the rate of respiration and changes in median cell volume in response to nutritional upshifts at different times of starvation were examined. The relative rates of RNA and protein synthesis increased immediately upon addition of glucose minimal medium; the increase in protein synthesis was not dependent on a parallel increase in RNA synthesis, indicating that the starved cells may have an excess of protein synthesizing machinery, including stable RNA and functional ribosom?. The subsequent increase in the rate of DNA replication was initiated approximately 60min before the first apparent cell division at approximately one doubling of the theoretical minimal cell volume ( Vu). Two-dimensional gel electrophoresis was used to demonstrate the fate of starvationspecific proteins during the upshift, and also the synthesis of recovery-induced proteins that were not found in starving cells. Most starvation-inducible proteins were repressed immediately at the onset of the nutritional upshift, while 11 of the 21 recovery-induced proteins identified were expressed exclusively during the maturation phase and were subsequently repressed at the onset of regrowth. The possible role of such maturation-specific proteins in the rapid formation of a reproductive cell committed to growth and division is discussed.

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“…strain S14 that are starved for a long time (120 h) are not dormant. These cells possess highly efficient transport systems (4,31) and synthesize protein at low, but easily detectable rates (45,49). The metabolically active ultramicrocells are capable of rapidly recovering from starvation and respond instantaneously to the addition of nutrients by increasing the rates of RNA synthesis and protein synthesis severalfold (3).…”

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Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956

1992

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Synthesis of Membrane and Periplasmic Proteins during Starvation of a Marine Vibrio sp.

Cited by 31 publications

References 21 publications

Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean

Comparison of DNA and protein synthesis rates of bacterial assemblages between coral reef waters and pelagic waters in tropical ocean

Macromolecular synthesis during recovery of the marine Vibrio sp. S14 from starvation

Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956

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