Synthesis of Magnetic Nanoparticles Stabilized by Magnetite-Binding Protein for Targeted Delivery to Cancer Cells

Abstract

A new method for obtaining biomodified magnetite nanoparticles for targeted delivery to cells was developed. The method is based on the use of the C-terminal fragment of the Mms6 protein, which is involved in the magnetite biomineralization during the synthesis of magnetosomes in magnetotactic bacteria Magnetospirillum magneticum AMB-1, and the barnase*barstar high-affinity protein pair. The Mms6 protein fragment is required for stabilizing magnetite, and the barnase*barstar pair mediates the interaction between nanoparticles and the component for modification. The efficiency of this method was confirmed in the synthesis of magnetite nanoparticles recognizing the HER2/neu tumor marker and in the selective labeling of HER2/neu with these nanoparticles on the surface of cancer cells.

Magnetic Iron Oxide Nanoparticles: Synthesis, Characterization and Functionalization for Biomedical Applications in the Central Nervous System

“…The selected literature was classified into four main groups:Methods of synthesis of IONPs [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56]Characterization of IONPs [57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,…”

“…Methods of synthesis of IONPs [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56]…”

“…In particular, some species are able to produce “biogenic” magnetic nanoparticles on which they base their sense of direction. For example, magnetotactic bacteria can generate magnetosomes (protein-coated nanosized crystals of magnetic iron oxide) [43,44]. For the typical parameters (time, temperature, and MNP size) characterizing these bio-mineralization processes, see Table 1.…”

Microbial Fabricated Nanosystems: Applications in Drug Delivery and Targeting

“…Thus, instead of chemical modifications on the magnetosome that might exert toxic effects, genetic engineering modifications allow expression of functional proteins on the magnetosome membrane. In a recent experiment, a biomodified magnetite nanoparticle was developed using the Mms6 protein and the barnase * barstar high-affinity protein pair to efficiently recognize and target HER2/neu tumor marker on the surface of cancer cells (Kotelnikova et al, 2018). In another interesting experiment, unmodified magnetotactic bacteria was formulated as DNA carriers using AuNP as a transmembrane facilitator to endocytosis.…”

PLGA Nanoparticles Decorated with Anti-HER2 Affibody for Targeted Delivery and Photoinduced Cell Death

“…However, the use of IgG for the delivery of nanopreparations has many serious drawbacks: high immunogenicity, the unoriented conjugation of molecules to the surface of the nanoparticle, and the need for the production of molecules in mammalian cell cultures due to the necessary post-translational modifications. Artificial scaffold polypeptides, which have already proven themselves as promising instruments for nanoparticle delivery, are an excellent alternative to full-length IgGs [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. Such polypeptides, for example, DARPins and affibodies, are obtained by the method of ribosomal or phage display, and have dissociation constants in the picomolar range for certain specific targets.…”