Synthesis of macroporous polymer rods based on an acrylamide derivative monomer

Abstract

New macroporous polymer rods were prepared by free-radical crosslinking copolymerization from N-acryloyl-tris(hydroxymethyl)aminomethane and N,N 0methylenebisacrylamide as a crosslinking agent with different porogenic mixtures and with azobisisobutyronitrile as an initiator. The porous properties of these materials were controlled through changes in the proportions of the porogenic mixture, the polymerization temperature, or the concentration of the crosslinking agent. Pore size distribution profiles that shifted toward a larger pore size were obtained in the following cases: when the percentage of the coporogen was increased, when the copolymerization reactions were carried out at a low temperature (55 8C), and when the crosslinking concentration was reduced. Alternatively, a porogenic mixture formed from dimethyl sulfoxide and a 1:1 combination of tetradecanol and poly(ethylene glycol) 6000 as coporogens yielded a polymer rod with a high porosity and pore size. These hydrophilic materials are promising as base supports for different chromatographic processes and as throughput bioreactors. V V C 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: [6616][6617][6618][6619][6620][6621][6622][6623] 2006

Macroporous bead modification with polyethylenimines of different molecular weights as polycationic ligands

“…In this study, Ech was used as an activator agent for the attachment of PEI to the beads and spacer arm between the surface of the beads and the functional groups of PEI. The activation of the hydroxyl groups of beads with Ech was carried out according to the experimental conditions previously studied, which yielded satisfactory results 21, 22, 25. This reaction yielded 0.3 mmol of epoxy groups/g of dry polymer, for which the density of epoxy groups on the porous surface was 9.29 μmol/m 2 and the estimated reaction efficiency was 5.4%.…”

“…One of the topics of discussion within our research group5, 20–26 is the synthesis of macroporous matrices that provide surface area for chemical modification to reach efficient adsorbents for affinity chromatography and that allow internal diffusion of great size solutes. On this occasion, the poly(HEMA‐ co ‐EGDMA) beads, with an initial molar ratio of 4 : 1 of HEMA : EGDMA in the medium of the reaction, were synthesized by suspension polymerization to generate porous crosslinked spherical beads.…”

Poly(ethylene glycol)‐based monolithic capillary columns for hydrophobic interaction chromatography of immunoglobulin G subclasses and variants

“…The polymerization mixture used for the synthesis of all PEGDA monoliths in this work contained 40 wt% PEGDA 258 with 40 wt% PEG 10 000 (20 wt% in methanol) and 20 wt% decanol. PEG, as a biocompatible polymer, has demonstrated effectiveness in influencing the morphology and pore size of the monoliths when used as a porogen. In this work, methanol was used for the dissolution of PEG and was found to result in a larger pore size in comparison to 2‐methoxyethanol, which was used previously as a solvent (see Supporting Information Fig.…”

Macroporous Monolithic Polymers: Preparation and Applications

“…A decrease in the percentage of the cross-linking agent in the reaction mixture to yield monoliths leads to a shift in the pore size distribution curve toward larger pore sizes [36]. This behavior could be due to the fact that an increase in the cross-linking agent concentration could lead to the formation of more cross-linked nuclei in the early stage of the polymerization reaction allowing an earlier separation phase.…”