Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

Namiki, Yoshihisa

doi:10.1007/978-1-62703-140-0_16

Cited by 4 publications

(1 citation statement)

References 7 publications

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“…For use in therapy, efficient delivery methods need to be developed. There are current efforts to develop adeno-associated virus vectors (Ketzer et al 2012), cationic peptides (Ewert et al 2008), nanoparticles (Chen et al 2012;Namiki 2013), and modified Salmonella strains (Yuhua et al 2001;Bai et al 2011) as delivery vehicles, yet improvements to minimize toxicity and immune responses and increase delivery to target tissues are still needed. Additionally, for the use as a tool in therapy or research, improvements are required to increase splicing efficiency in cells, and reduce off-target effects.…”

Section: Introductionmentioning

confidence: 43%

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation

Amini

Müller

2015

RNA

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The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the spliceosome. These spliceozymes were improved in this study by 10 rounds of evolution in Escherichia coli cells. One clone with increased activity in E. coli cells was analyzed in detail. Three of its 10 necessary mutations extended the substrate binding duplexes, which led to increased product formation and reduced cleavage at the 5 ′ -splice site. One mutation in the conserved core of the spliceozyme led to a further reduction of cleavage at the 5 ′ -splice site but an increase in cleavage side products at the 3 ′ -splice site. The latter was partially reduced by six additional mutations. Together, the mutations increased product formation while reducing activity at the 5 ′ -splice site and increasing activity at the 3 ′ -splice site. These results show the adaptation of a ribozyme that evolved in nature for cis-splicing to transsplicing, and they highlight the interdependent function of nucleotides within group I intron ribozymes. Implications for the possible use of spliceozymes as tools in research and therapy, and as a model for the evolution of the spliceosome, are discussed.

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Section: Introductionmentioning

confidence: 43%

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation

Amini

Müller

2015

RNA

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Magnetic hydrogel for the rapid elimination of environmental harmful ions

Namiki¹

2020

J Clin Microbiol Biochem Technol

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Aqueous solution containing selenium (1 mg/mL), boron (3 mg/mL), fl uorine (3 mg/mL), and arsenic (1 mg/mL), and magnetic hydrogel (15 w/w%) was mixed. After 24 hours of mixing, magnetic hydrogel was removed using magnet (0.5 tesla) for 20 seconds, we measured the concentration of selenium, boron, fl uorine and arsenic. The average concentration of them was 0.009 mg/mL, 0.07 mg/mL, 0.10 mg/mL and 0.004 mg/mL, respectively. Finally, we confi rmed

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Rapid and Reliable Elimination System for Radioactive Cesium Using Magnetic Nanocomposites

Namiki

2014

J. Jpn. Soc. Powder Powder Metallurgy

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We have devised the rapid elimination system for the contaminated radioactive cesium using magnetic nanocomposites. The Great East Japan Earthquake hit caused a serious meltdown of the overheated fuel rods and the release of radioactive chemicals into the environment. Until now, fuel rods have been cooled using seawater to control its temperature, therefore huge amount of waste water containing high concentrations of radioactive cesium have been continuously generated. On the other hand, radioactive cesium is easily condensed in the fly ash originating from incineration of garbage. The garbage incineration plant is overflowing with the fly ash which cannot be discarded (when the radioactivity of fly ash exceeds 8000 Bq/kg, we cannot discard it as a usual manner). Prussian blue is known as an effective eliminator of radioacitive cesium from seawater and fly ash slurry, however complete and rapid collection of Prussian blue dispersed in water is very difficult. Recently, we have developed a novel form of magnetically guidable Prussian blue coated magnetic nanocomposite clusters via the application of nanotechnology for drug delivery system. Using this type of nanocomposites under magnetic field, we have confirmed that 99.9% and over 80% of cesium were rapidly removed from seawater and fly ash, respectively.

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Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

Cited by 4 publications

References 7 publications

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation

Magnetic hydrogel for the rapid elimination of environmental harmful ions

Rapid and Reliable Elimination System for Radioactive Cesium Using Magnetic Nanocomposites

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