Synthesis of Isotopically Labeled 13C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil

Abstract

Abstract:The isotope dilution mass spectrometry (IDMS) is a highly efficient method for tackling the ion suppression in complex matrix by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), but a lack of commercial internal standards is a limiting factor for these analyses. Herein, an economical and efficient strategy for the synthesis of 13 C 3 -simazine via a three-step procedure was developed. The isotope-labeled internal standard was used for determination of simazine residue in soil samples. The quantitation method has a limit of detection of 0.015 µg/kg and quantitation of 0.08 µg/kg. The inter-day and intra-day precision of the method were below 4.6%. Recovery values were ranged between 92.9% and 99.2%. All the samples obtained from six provinces in China contained from 1 to 62 µg/kg of simazine.

Ultra Performance Liquid Chromatography (UPLC) - A Review

“…The calcium carbonate column serves as an adsorbent, and the various compounds are adsorbed to varying degrees, resulting in coloured bands at various positions on the column. The Greek terms chroma and graphos, which imply colour and writing, respectively, inspired Tswett to use the term "chromatogram" to describe this system of coloured bands and the technique used to generate it [13][14][15][16][17][18][19]. Since then, significant progress has been made, and the techniques are now employed to differentiate coloured and colourless compounds.…”