Synthesis of Isotope‐Labeled Deoxynivalenol‐15‐O‐Glycosides

Abstract

A versatile and efficient protocol for the Schmidt glycosylation of 3‐acetyldeoxynivalenol (3‐ADON) and subsequent deprotection has been developed to gain access to deoxynivalenol‐15‐O‐glycosides in reasonable amounts for biological analysis and further investigations. By using this protocol with [13C6]glycosyl donors, we were able to prepare isotope‐labeled deoxynivalenol‐15‐O‐glycosides, which are pivotal to enabling accurate quantification of masked mycotoxins by LC–MS.