Synthesis of (Z)‐3‐hexen‐1‐yl acetate by lipase immobilized in polyvinyl alcohol nanofibers

Abstract

Polyvinyl alcohol (PVA)-nanofibers-immobilized lipase were formed by electrospinning. The specific surface area of the nanofiber (5.96 m 2 /g) was about 250 times larger than that of PVA-film-immobilized lipase (0.024 m 2 /g). The PVA-nanofibers-immobilized lipase were used as the catalyst for the esterification of (Z )-3-hexen-1-ol (leaf alcohol) with acetic acid in hexane. The activity of the nanofiber is equivalent to that of commercially available immobilized lipase (Novozym-435). The ester conversions of the nanofibers, Novozym-435, the film and lipase powder reached 99.5% at 5 h, 100% at 5 h, 11.5% at 6 h, and 81.1% at 5.75 h, respectively. The nanofibers-immobilized lipase showed higher activity for the esterification than the film-immobilized lipase and lipase powder, probably because it has high specific surface area and high dispersion state of lipase molecules in PVA matrix.

Preparation and characterization of gelatin hydrogel support for immobilization ofCandida Rugosalipase

“…This result is due to the inactivation of the enzyme caused by the denaturation of protein. The similar behavior of immobilized enzyme was reported in the literature (Nakane et al 2007). …”

Applications of electrospun nanofibers

“…To immobilize enzyme on electrospun nanofibers, many approaches have been used [217][218][219][220][221][222][223][224][225][226][227][228][229] , including grafting enzyme on fiber surface, physical adsorption, and incorporating enzyme into nanofiber via electrospinning followed by crosslinking reaction.…”

Enhanced productivity of electrospun polyvinyl alcohol nanofibrous mats using aqueous N,N-dimethylformamide solution and their application to lipase-immobilizing membrane-shaped catalysts

“…In the current study, this system for the enhanced electrospun PVA fiber production was applied to lipase-immobilizing PVA fibrous catalysts, according to previous reports indicating the adaptability of electrospun PVA fibers as lipase carriers for reactions in organic solvents (6,27,28). First, the catalytic activity of immobilized lipase was investigated by measuring the initial reaction rate of the transesterification of (S)-glycidol to (S)-glycidyl n-butyrate in isooctane.…”