Synthesis of hybrid myocardium constructs and in vitro characterization under mechanical stimulation

Extracellular Matrix-Based Conductive Composites for Myocardial Tissue Regeneration

“…at the end of the first day, fourth day, seventh day, and 14th day, respectively (Alamar blue analysis of the hybrid form “composed of decellularized ECM and PGS together” of these composites was presented in our previous study, and the absorbance values were determined as 0.06 ± 0.001 O.D., 0.09 ± 0.07 O.D., 0.16 ± 0.007 O.D., and 0.20 ± 0.007 O.D. at the end of the first day, fourth day, seventh day, and 14th day, respectively . The increase in the metabolic activities of the cells between the hybrid and composite patches was observed statistically up to fourth day ( p ≤ 0.05); however, from the seventh to the 14th day there was no statistical difference ( p > 0.05) between the hybrid and composite structures.…”

“…The thickness of the decellularized matrices obtained with the previous results 11 was specified as 1.90 ± 0.14 mm. The thickness of the composite structures obtained with the addition of PGS and carbon geometries in this study was calculated as 1.85 ± 0.22 mm (n.s.).…”

“…The conductivity properties of the recellularized composite structures were calculated with the current values recorded against the applied voltage values, and the resistance (ohm•m) and conductivity (Siemens/m) results are given in Figure 3. For comparative results, the control group (obtained by seeding an equal number of cells into an empty plate well), the recellularized hybrid structures 11 (composed of decellularized ECM and PGS together, the nondoped MWCNT form of composite), and the recellularized composite structures were included and discussed. Meanwhile, the current values of the cell-free scaffolds were recorded, and the results of the control group, hybrid structures, and composite structures were normalized accordingly.…”

“…MWCNTs were dispersed in the prepolymer in the synthesis step, and then the whole structure was cross-linked. The conductivity of the For the preparation of myocardial extracellular matrices, the previously published decellularization protocol by our group was applied 11 (the brief decellularization procedure was given in the Supporting Information). Following the decellularization process, PGS prepolymer was prepared in order to synthesize composite structures.…”

“…MWCNT impregnated prepolymers were kept in a vacuum oven (Memmert, Germany) at 150 °C for 1 h and allowed to form into liquid form. Finally, decellularized-lyophilized myocardial grafts 11 were embedded in liquid prepolymer solution and then cross-linked with a dehydrothermal approach (based on the forming of the ester linkages by carboxylic groups) at 8 mbar and 150 °C for 10 h. The conductivity and resistance properties, mechanical characteristics, and degradation behavior were determined. Micro-CT analysis and SEM images were presented; cell viability studies for the composite structures were evaluated.…”