Synthesis of Hybrid Chitosan Silver Nanoparticles Loaded with Doxorubicin with Promising Anti-cancer Activity

Mohamed, Noha

doi:10.1007/s12668-020-00760-y

Cited by 21 publications

(6 citation statements)

References 43 publications

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“…The increased behavior of the release of flavonoids at pH 5 is suitable for the tumor region. 29 There are various factors that can delay the release of plant extracts from the nanospheres. One factor can be the strong chemical interaction between the plant extract and the polymer, and another factor that can influence the plant extract release is the stiffening of the polymer network during the cross-linking of the nanoparticle process.…”

Section: Results and Discussionmentioning

confidence: 99%

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ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

2021

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In recent decades, biodegradable polymeric nanoparticles have been used as a nanocarrier for the delivery of anticancer drugs. In the present study, we synthesize bovine serum albumin (BSA) nanospheres and evaluate their ability to incorporate a plant extract with anticancer activity. The plant extract used was the methanol fruit extract of Cucumis prophetarum , which is a medicinal herb. The fruit-extract-encapsulated BSA nanospheres (Cp-BSA nanospheres) were prepared using a desolvation method at various pH values of 5, 7, and 9. The nanosphere formulations were characterized using various techniques such as dynamic light scattering (DLS), ζ-potential, Fourier transform infrared spectroscopy (FTIR), and field-effect scanning electron microscopy (FESEM). The results show that the Cp-BSA nanospheres prepared at pH 7 were spherical with a uniform particle size, low polydispersity index (PDI), ζ-potential, and high entrapment efficiency (82.3%) and showed sustained release of fruit extract from Cp-BSA nanospheres in phosphate-buffered saline (PBS), pH 5. The anticancer activity was evaluated on A549, HepG2, MCF-7 cancer cell lines and HEK 293 normal cell lines. In vitro, antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, intracellular reactive oxygen species (ROS) production, and mitochondrial membrane potential were estimated. An in vitro cellular uptake study was performed using fluorescein isothiocyanate (FITC) dye at a different time of incubation, and DNA fragmentation was observed in a dose-dependent manner. The gene expression level of Bax and the suppression level of Bcl-2 were observed upon the treatment of Cp-BSA nanospheres. Thus, the Cp-BSA nanospheres triggered ROS-dependent mitochondrial apoptosis in different human cancer cell lines when compared to the noncancerous cell lines and could be used as a potential candidate for anticancer agents.

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Section: Results and Discussionmentioning

confidence: 99%

“…The total release rates of flavonoids from Cp-BSA NPs for over 72 h at 5, 7, and 9 were 76, 64, and 39%, respectively. The increased behavior of the release of flavonoids at pH 5 is suitable for the tumor region . There are various factors that can delay the release of plant extracts from the nanospheres.…”

Section: Results and Discussionmentioning

confidence: 99%

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

2021

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“…Ag NPs can also be conjugated and loaded with other agents and drugs to make their delivery more efficient and effective. In one study by Mohamed (2020), silver chitosan NPs were prepared and loaded with doxorubicin. The doxorubicin release was evaluated at different pH to investigate the efficacy of Ch-AgNPs-DOX.…”

Section: Silver Npsmentioning

confidence: 99%

“…Results also clearly indicated that Ch-AgNPs-DOX could show a massive area of apoptotic cells. Hence the efficacy of the transport of DOX drug and efficient release of the drug by Ch-AgNPs were proven to be high (Mohamed 2020). More studies of silver NPs' effect on cancer are depicted in table 2inorganic NPs.…”

Section: Silver Npsmentioning

confidence: 99%

Current development of theragnostic nanoparticles for women’s cancer treatment

Kashyap,

Kumari,

Singh

et al. 2024

Biomed. Mater.

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In the biomedical industry, nanoparticles (NPs - exclusively small particles with size ranging from 1 – 100 nanometres) are recently employed as powerful tools due to their huge potential in sophisticated and enhanced cancer theragnostic (i.e. therapeutics and diagnostics). Cancer is a life-threatening disease caused by carcinogenic agents and mutation in cells, leading to uncontrolled cell growth and harming the body's normal functioning while affecting several factors like low levels of ROS, hyperactive antiapoptotic mRNA expression, reduced proapoptotic mRNA expression, damaged DNA repair, and so on. NPs are extensively used in early cancer diagnosis and are functionalized to target receptors overexpressing cancer cells for effective cancer treatment. This review focuses explicitly on how NPs alone and combined with imaging techniques and advanced treatment techniques have been researched against “women’s cancer” such as breast, ovarian, and cervical cancer which are substantially occurring in women. NPs, in combination with numerous imaging techniques (like PET, SPECT, MRI, etc.) have been widely explored for cancer imaging and understanding tumor characteristics. Moreover, NPs in combination with various advanced cancer therapeutics (like magnetic hyperthermia, pH responsiveness, photothermal therapy, etc.), have been stated to be more targeted and effective therapeutic strategies with negligible side effects. Furthermore, this review will further help to improve treatment outcomes and patient quality of life based on the theragnostic application-based studies of NPs in women’s cancer treatment.

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“…This qualifies it for pharmaceutical and biological uses, [15] including medication delivery [16] . Combining the characteristics of silver and chitosan to generate nanohybrids is particularly promising for antibacterial and anti‐cancer applications [17] …”

Section: Introductionmentioning

confidence: 99%

Effect of Chitosan‐Silver Nanoparticles on DEN‐induced Hepatocellular Injury by Regulation of Wnt/β‐Catenin and Apoptosis Signaling Pathways in Rats

Nasef,

Mohamed,

Mansour

2024

ChemistrySelect

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Silver nanoparticles (AgNPs) have gained remarkable attention as therapeutic agents since the appearance of nanotechnology. This study aims to assess the protective impact of chitosan‐silver nanoparticles (CS‐AgNPs) against DEN‐induced hepatocellular injury by regulation of Wnt/β‐Catenin and apoptosis signaling pathways in rats. CS‐AgNPs solutions were prepared by mixing different concentrations of chitosan and silver nitrate, and then subjected to gamma radiation at 20 kGy. The formation of CS‐AgNPs could be observed by naked eyes from the colour change from light yellow to reddish‐brown, and confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Stability measurements confirmed that the prepared Cs‐AgNPs are highly stable for around 8 weeks. A crystal violet cytotoxicity assay of CS‐AgNPs has been detected in vitro on HepG2 hepatocellular carcinoma cell lines. The obtained results confirmed the selective influence of CS‐AgNPs on the HCC cell line in a time and dose dependent manner. Diethylnitrosamine (DEN) induced a marked increase in the expressions of Wnt, β‐Catenin, STAT3, MMP9, and Bcl‐2 with a significant decrease in the expression of BAX, p53, and P21. Additionally, DEN significantly decreased the levels of Cytc and TPTH while significantly increased the levels of COX‐2, PGE2, TGF‐1, AFP, and liver enzyme activity. The role of CS‐AgNPs is documented in diminishing the expressions of Wnt, β‐Catenin, STAT3, MMP9, and Bcl‐2. Also, CS‐AgNPs induced the decrease in the levels of COX‐2, PGE2, TGF‐1, AFP and liver enzyme activity accompanied by increased apoptosis. These results concluded that CS‐AgNPs might be a promising drug designed for treating liver diseases.

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Synthesis of Hybrid Chitosan Silver Nanoparticles Loaded with Doxorubicin with Promising Anti-cancer Activity

Cited by 21 publications

References 43 publications

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

ROS-Mediated Apoptosis Induced by BSA Nanospheres Encapsulated with Fruit Extract of Cucumis prophetarum in Various Human Cancer Cell Lines

Current development of theragnostic nanoparticles for women’s cancer treatment

Effect of Chitosan‐Silver Nanoparticles on DEN‐induced Hepatocellular Injury by Regulation of Wnt/β‐Catenin and Apoptosis Signaling Pathways in Rats

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