Synthesis of Heparin-Like Glycosaminoglycans in Rat Ovarian Slices1

Gebauer, H.; Lindner, H. R.; Amsterdam, Abraham

doi:10.1095/biolreprod18.3.350

Cited by 70 publications

(25 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ref. 43) (47), and it was suggested that transmembrane proteoglycans may interact with cytoskeletal elements that terminate at the plasma membrane (48,49).…”

Section: Discussionmentioning

confidence: 99%

Regulation of cytoskeletal proteins involved in cell contact formation during differentiation of granulosa cells on extracellular matrix.

Ben-Ze’ev¹,

Amsterdam²

1986

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The organzation and the expression of cytoskeletal proteins involved in determining cell contact and shape were analyzed in granulosa cells during their differentiation on extracellular matrix (ECM)-coated culture plates. Rat granulosa cells from preovulatory follicles displayed an epithelial shape on ECM and formed multilayered aggregates with numerous gap junctions between neighboring cells. These cells had few actin cables and often only a diffuse pattern of actin and a low amount of vinculin in very thin focal adhesion sites. In contrast, cells grown on plastic formed a monolayer of fiat cells with a reduced number of gap junctions but with numerous stress fibers and abundant large vinculin-containing focal contacts. On ECM, the cells were stimulated to produce high levels of progesterone, while only trace amounts of the steroid accumulated in cells on plastic dishes. Two-dimensional gel electrophoretic analysis of [35Smethionine-labeled cells revealed a dramatic decrease in vinculin, a-ain, and actin synthesis in cells grown on ECM, as compared to cells grown on plastic, while the synthesis of the tubulins and of the intermediate filament protein vimentin remained unchanged. RNA blot analysis showed a marked decrease in actin mRNA levels in cells from ECM plates, while the level of tubulin mRNA remained essentially unchanged. It is concluded that the differentiation of granulesa cells on ECM in vitro is associated with changes in cell shape and cell contacts add that such changes in cell morphology are accompanied by simultaneous alterations in the organization and expression of cytoskeletal proteins that are involved in determining these cellular structures.Numerous lines of evidence suggest that important growthrelated cellular functions such as cell proliferation (1), expression of the differentiated phenotype (2-7), response to tissue-specific hormones (8), and tissue morphogenesis during embryonic development (9, 10) are modulated through changes in cell shape (for reviews, see refs. 11 and 12). Since cytoskeletal elements are responsible for determining cell morphology and shape, our studies on cell configurationrelated gene expression (13)(14)(15)(16)(17) were directed toward investigating the possible existence of a linkage between the expression of genes coding for cytoskeletal proteins and the organization in the cell of the respective proteins (for reviews, see refs. 18 and 19).The extracellular matrix (ECM), a natural substrate produced by cells, affects cell proliferation, migration, and differentiation in vivo (20)(21)(22)(23). The culturing of cells on ECM was shown to enhance cell differentiation (7, 9) and also to affect cell shape via reorganization of the cytoskeletal networks (24,25). In previous studies, it was found that granulosa cells associated with the basement membrane, in the preovulatory follicle, contained a higher density of receptors to luteinizing hormone and a higher steroidogenic activity as compared to more distal cell layers (26,27). In this report, we studied th...

show abstract

“…ref. 43) (47), and it was suggested that transmembrane proteoglycans may interact with cytoskeletal elements that terminate at the plasma membrane (48,49).…”

Section: Discussionmentioning

confidence: 99%

Regulation of cytoskeletal proteins involved in cell contact formation during differentiation of granulosa cells on extracellular matrix.

Ben-Ze’ev¹,

Amsterdam²

1986

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…GAGs, synthesized by granulosa and theca cells, are present in the uterine and follicular fluids [2,8,201. They are also present in the cumulus-oocyte complex and in the zona pellucida, as well as in the oocyte cytoplasm and in the secretion of the cortical granules of the ovum [6, 261.…”

mentioning

confidence: 99%

Differential Decondensation of Class I (Rat) and Class II (Mouse) Spermatozoa Nuclei by Physiological Concentrations of Heparin and Glutathione

Sánchez-Vázquez

Reyes

Delgado

et al. 1996

Archives of Andrology

View full text Add to dashboard Cite

“…The present findings that heparin inhibits testicular responsiveness to LH in vitro and the observation that sulfated mucopolysaccharides may be involved in regulation of the ovulatory process in rodents [4,16] indicate the need for further study of the possible effects of these compounds on gonadal function. Studies are currently in progress to investigate the possible effects of a variety of glycosaminoglycans on testicular adenylate cyclase activity and testosterone production in response to LH stimulation.…”

Section: Resultsmentioning

confidence: 74%

The influence of heparin on LH‐ and dibutyryl‐cAMP‐stimulated testosterone production in vitro by adult rat testes

Fox

Wisner

1979

FEBS Letters

View full text Add to dashboard Cite

Synthesis of Heparin-Like Glycosaminoglycans in Rat Ovarian Slices1

Cited by 70 publications

References 24 publications

Regulation of cytoskeletal proteins involved in cell contact formation during differentiation of granulosa cells on extracellular matrix.

Regulation of cytoskeletal proteins involved in cell contact formation during differentiation of granulosa cells on extracellular matrix.

Differential Decondensation of Class I (Rat) and Class II (Mouse) Spermatozoa Nuclei by Physiological Concentrations of Heparin and Glutathione

The influence of heparin on LH‐ and dibutyryl‐cAMP‐stimulated testosterone production in vitro by adult rat testes

Contact Info

Product

Resources

About