Synthesis of glycoproteins in cells infected by the flavivirus Kunjin

Wright, P.J.; Warr, Heather M; Westaway, E. G.

doi:10.1016/0042-6822(81)90512-2

Cited by 29 publications

(35 citation statements)

References 36 publications

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“…These results were similar to those reported for gp66 and gp53 of KUN (Wright et al, 1981), and were suggestive of a precursor-product relationship between the two WN glycoproteins. The proportion of gp58 relative to gp54 detected in WN virus-infected cells was less than the proportion of gp59 relative to gp53 observed in cells infected by KUN virus (Wright et al, 1981). No conclusions were possible concerning the involvement of WN gp58 either as a precursor of gp66 or gp54, or as an intermediate during processing of gp66.…”

supporting

confidence: 89%

“…The strains of KUN and WN viruses used in these studies, and the procedures followed to radiolabel virions and infect Vero cells have been described previously (Wright et al, 1981;Wright, 1982). Proteins were separated in polyacrylamide gels containing SDS using a discontinuous buffer system (Laemmli, 1970).…”

Section: Discussionmentioning

confidence: 99%

“…Formerly, we showed by radioimmunoprecipitation using antiserum against E, and by limited proteolytic digestion, that KUN virus-infected Vero cells contain at least two glycoproteins related to E of KUN virus, namely gp53 and gp59. A third glycoprotein gp66 is an apparent precursor to gp53 in pulse-chase experiments, but is not immunoprecipitated by antiserum against E. Limited proteolytic digestion of [3H]mannose-labelled gp53, gp59 and gp66 suggested that gp66 is probably related to the other two glycoproteins, but the results were not conclusive (Wright et al, 1981). Therefore, to confirm the relationship, we have now analysed the peptides produced by exhaustive proteolytic digestion of the [3H]leucine-labelled glycoproteins.…”

mentioning

confidence: 99%

“…In this paper, the two WN glycoproteins are compared with each other and with E of WN virus by peptide mapping. These experiments were undertaken to determine whether KUN virus is unique among flaviviruses in specifying more than one intracellular E-related glycoprotein, or whether the presence of such glycoproteins is a more general characteristic of flavivirus-infected cells.The strains of KUN and WN viruses used in these studies, and the procedures followed to radiolabel virions and infect Vero cells have been described previously (Wright et al, 1981;Wright, 1982). Proteins were separated in polyacrylamide gels containing SDS using a discontinuous buffer system (Laemmli, 1970).…”

mentioning

confidence: 99%

“…KUN virus-specified glycoproteins are analysed in lane 6. For consistency, previously assigned molecular weights and names have been given to polypeptides already described Wright et al, 1981). The approximate molecular weights of the polypeptides in the gel system used in Fig.…”

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confidence: 99%

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Peptide Mapping of Envelope-related Glycoproteins Specified by the Flaviviruses Kunjin and West Nile

Wright

Warr

1985

Journal of General Virology

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SUMMARYGlycoproteins detected in Vero cells infected by the flaviviruses West Nile and Kunjin were examined by gel electrophoresis and peptide mapping. Two major glycoproteins, gp66 and gp54, were observed in West Nile virus-infected cells labelled for short time periods with [3H]mannose. A third glycoprotein, gp58, was present in smaller amounts. Pulse-labelling experiments suggested that gp66 was a precursor of gp54. Peptide mapping of [3H]leucine-labelled gp66, gp54 and the envelope glycoprotein E of West Nile virus demonstrated that gp66 and gp54 were related to E, and that the peptides of gp54 were a subset of those of gp66. Peptide mapping of the corresponding Kunjin virus-specified glycoproteins (gp66, gp59 and gp53) showed that the [3H]leucine-labeUed peptides of gp53 and gp59 were similar and were contained within gp66. Since we have shown previously that gp59 and gp53 are related to E of Kunjin virus, we conclude that cells infected by West Nile or Kunjin viruses contain a similar set of E-related glycoproteins.Kunjin (KUN) and West Nile (WN) viruses belong to the same serological complex in the flavivirus genus within the family Togaviridae. Virions contain genomic RNA of positive polarity, two small polypeptides C and M, and one envelope protein E (for review, see Russell et al., 1980). Formerly, we showed by radioimmunoprecipitation using antiserum against E, and by limited proteolytic digestion, that KUN virus-infected Vero cells contain at least two glycoproteins related to E of KUN virus, namely gp53 and gp59. A third glycoprotein gp66 is an apparent precursor to gp53 in pulse-chase experiments, but is not immunoprecipitated by antiserum against E. Limited proteolytic digestion of [3H]mannose-labelled gp53, gp59 and gp66 suggested that gp66 is probably related to the other two glycoproteins, but the results were not conclusive (Wright et al., 1981). Therefore, to confirm the relationship, we have now analysed the peptides produced by exhaustive proteolytic digestion of the [3H]leucine-labelled glycoproteins.In cells infected by WN virus, two glycoproteins similar in size to gp66 and gp53 specified by KUN virus were observed, but their relationships to E were not examined (Wright, 1982). In this paper, the two WN glycoproteins are compared with each other and with E of WN virus by peptide mapping. These experiments were undertaken to determine whether KUN virus is unique among flaviviruses in specifying more than one intracellular E-related glycoprotein, or whether the presence of such glycoproteins is a more general characteristic of flavivirus-infected cells.The strains of KUN and WN viruses used in these studies, and the procedures followed to radiolabel virions and infect Vero cells have been described previously (Wright et al., 1981;Wright, 1982). Proteins were separated in polyacrylamide gels containing SDS using a discontinuous buffer system (Laemmli, 1970). To obtain suitable preparations of the intracellular viral glycoproteins for peptide mapping, infected cells were labelled with [3H]le...

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supporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Peptide Mapping of Envelope-related Glycoproteins Specified by the Flaviviruses Kunjin and West Nile

Wright

Warr

1985

Journal of General Virology

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Synthesis of human rotavirus polypeptides in cell culture

Heath

Birch

1988

Journal of Medical Virology

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The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of infection of the virus, incorporation of actinomycin D and excess NaCl into the medium resulted in significant reduction in host cell background and enabled observation of viral polypeptides as early as 2 hours post infection. Five polypeptides were found to be structural components of the virion, and a further eight appeared to be nonstructural proteins or intermediates. Five polypeptides were glycosylated during virus replication, but only one of these, VP7, was a definite structural glycoprotein. Pulse-chase experiments revealed that four low molecular weight polypeptides underwent post-translational modifications.

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