Synthesis of glycinamides using protease immobilized magnetic nanoparticles

Abstract

HighlightsProtease producing Bacillus subtilis was isolated from animal slaughter house waste.Protease was covalently immobilized on amino-functionalized magnetic nanoparticles (AMNPs).To our knowledge, for the first time these magnetic nano biocatalyst was used for the synthesis of series of novel glycinamides.Parameters for glycinamides synthesis such as, pH, temperature and time were optimized using Response Surface Methodology.Reusability study showed that protease immobilized MNPs retain up to 70% of initial activity after 8th cycles of reuse for the synthesis.

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

“…where Y is the predicted response used as a dependent variable, X i and X j are the levels of variables, β 0 is the constant term, β i is the coefficient of the linear terms, β ii is the coefficient of the quadratic terms, and β ij is the coefficient of the cross-product terms [15]. The amount of immobilized protein on the magnetite composite (Fe 3 O 4 @Chitosan@Proteins) was determined by subtracting the final from the initial concentration of protein in the immobilization medium.…”

“…The mass balance of protein calculated with TGA data was confirmed on the basis of the amount of proteins added and amount of proteins immobilized using Bradford assay (data not shown). TGA is frequently used to confirm the immobilization of enzymes on MNPs by determining the percentage loss of weight of the naked MNPs and enzyme immobilized MNPs [15]. The XRD patterns of magnetic composites are shown in Figure 5.…”

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources

“…The immobilisation yield (%) and the amount of immobilised protease (Phw_im, Psr_im) loading on MNPs (U/mg) were calculated using following equations (Eqs. 1, 2) [15,23]:…”

“…The use of MNPs has been particularly attractive for immobilisation because of their special characteristics, such as their high surface area, simple manipulation and separation by the application of an external magnetic field, biocompatibility, biodegradability, and low toxicity [10,14]. There are several studies reporting the application of purified protease immobilised on magnetic supports [15][16][17][18];…”

Non-covalent conjugation of cutinase from Fusarium sp. ICT SAC1 with pectin for enhanced stability: Process minutiae, kinetics, thermodynamics and structural study

“…Since the last few decades, enzymes have been extensively used as a catalyst in chemical, biotechnological and allied industrial sectors for numerous applications due to their high selectivity, sustainability, high efficiency and specificity, and their eco-friendly nature making the process green and clean [1,2]. Enzymes are becoming a vital tool in developing novel manufacturing processes for several industrial approaches, and hence garnered the focus of numerous researchers to utilize them.…”