“…Molecular biology and TEVC experiments were conducted as previously described. [ 25 , 26 , 27 ] Measurements were performed using a Turbo Tec 10CX amplifier (NPI electronic, Tamm, Germany), NI USB 6221 DA/AD Interface (National Instruments, Austin, USA) and GePulse Software (Dr. Michael Pusch, Genova, Italy). All experiments were conducted at room temperature.…”