Synthesis of gadolinium oxide nanocuboids for <i>in vitro</i> bioimaging applications

Chawda, Nitya; Mishra, Sarthak; Basu, Mainak; Chander, Harish; Podder, Raju; Mahapatra, S. K.; Banerjee, Indrani

doi:10.1088/2053-1591/ab1d24

Cited by 5 publications

(6 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the pathway of cellular internalization of nanoclusters is not explored very well, but few findings from previous research suggested that the involvement of cell-receipted medium form endocytosis is very much responsible for the internalization of prepared nanoclusters. [81,82] This In-vitro fluorescence imaging study revealed the efficacy of prepared Gd 2 O 3 nanoclusters in bioimaging applications. Additionally, the obtained image illustrates that the cell morphology remains uninfluenced after treatment with the Gd 2 O 3 nanocluster, which validated the noncytotoxic nature of Gd 2 O 3 nanoclusters…”

Section: In Vitro Imaging Of Gd 2 O 3 In Hacat Cell Linementioning

confidence: 75%

“…Microscopic image of Gd 2 O 3 nanocluster incubated HaCaT cells revealed diffused emission in the red, green, and blue channel after 24 hrs incubation with 1/10 th concentration of Gd 2 O 3 nanocluster (35 mg/ml stock), which demonstrated homogeneous internalized and cytoplasmic accumulation. However, the pathway of cellular internalization of nanoclusters is not explored very well, but few findings from previous research suggested that the involvement of cell‐receipted medium form endocytosis is very much responsible for the internalization of prepared nanoclusters [81,82] . This In‐vitro fluorescence imaging study revealed the efficacy of prepared Gd 2 O 3 nanoclusters in bioimaging applications.…”

Section: In Vitro Imaging Of Gd2o3 In Hacat Cell Linementioning

confidence: 82%

See 1 more Smart Citation

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

et al. 2022

View full text Add to dashboard Cite

Fluorescent probes are highly desirable for accurate diagnosis and play a crucial role in the optical imaging modality. Here, ultrafine (1.2 nm) nanoclusters of undoped gadolinium oxide were synthesized via a simple, one‐pot technique using water as the reaction medium. Prepared nanoclusters exhibit strong tuneable emission spanning from 400‐620 nm (extended visible region) with broad full width at half maximum of (FWHM) ∼140 nm, resulting in white light emission (WLE). Cytotoxicity studies revealed nearly 100 % cell viability encouraging its application in cell imaging. These nanoclusters possessed distinctive properties such as wide‐range pH stability, ionic tolerability, durable photostability, and anomalous colloidal stability (more than 24 months). This brief report uses white light‐emitting gadolinium oxide (Gd2O3) nanoclusters as an optical probe for in‐vitro fluorescence imaging.

show abstract

Section: In Vitro Imaging Of Gd 2 O 3 In Hacat Cell Linementioning

confidence: 75%

Section: In Vitro Imaging Of Gd2o3 In Hacat Cell Linementioning

confidence: 82%

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Manganese is an additional essential trace element in human bodies, but its acceptable limit is lower than that of yttrium. Other elements, such as Gd 3+ ions, are toxic in their free form, which necessitates proper coatings or chelating when they are used for biomedical applications 22 . In the present work, Yb 3+, and Er 3+ ions are embedded into the NaYF 4 host matrix, which lowers Yb 3+ and Er 3+ ions toxicity profile by confining them into the host material and lowering their release as ions make them benign in nature 23,24 …”

Section: Introductionmentioning

confidence: 87%

“…Other elements, such as Gd 3+ ions, are toxic in their free form, which necessitates proper coatings or chelating when they are used for biomedical applications. 22 In the present work, Yb 3+, and Er 3+ ions are embedded into the NaYF 4 host matrix, which lowers Yb 3+ and Er 3+ ions toxicity profile by confining them into the host material and lowering their release as ions make them benign in nature. 23,24 In the present work, NaYF 4 :Yb,Er has been synthesized successfully by the hydrothermal method and studied for their physicochemical characterization by powdered-X-ray diffraction (P-XRD), particle size analyzer (PSA), field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), luminescence study.…”

Section: Introductionmentioning

confidence: 89%

Ex vivo interaction study of NaYF₄:Yb,Er nanophosphors with isolated mitochondria

Singh

Chawda

Barkhade

et al. 2021

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

Ex vivo interaction of NaYF 4 :Yb,Er nanophosphors with isolated mitochondria has been investigated. The nanophosphors were synthesized using the hydrothermal method. The synthesized NaYF 4 :Yb,Er nanophosphors were characterized for physicochemical properties. The NaYF 4 :Yb,Er nanophosphors showed successful upconversion with excitation wavelength lying in the nearinfrared region. The effect of synthesized NaYF 4 :Yb,Er nanophosphors on mitochondria isolated from the chicken heart tissue was examined through ROS generation capacity, membrane fluidity, and complex II activity. The exposer of NaYF 4 :Yb,Er nanophosphors to isolated mitochondria inhibits ROS generation activity as compared to control. The mitochondria membrane fluidity of the lipid bilayer and complex-II activity of mitochondria was observed to be unaltered after the interaction with NaYF 4 :Yb,Er nanoparticles. The results confirm that synthesized NaYF 4 :Yb,Er nanoparticles can be used as a safe contrast agent.

show abstract

“…(𝛼ℎ𝑣) 𝑛 = 𝐶(ℎ𝑣 − 𝐸 𝑔 ) (4) where h is Planck's constant, ν is frequency of vibration, 𝐸 𝑔 is the value of band gap energy, C is a proportionality constant, n is the nature of transition and in this case n = 2 for direct band gap material. Traditionally, the energy band gap is obtained from the plot of   n hv R F ) ( vs hv by extrapolating the linear portion of the spectrum to the hv axis where   0 ) ( = n hv R F [58]; [57]. Selected Tauc's plots are given in Figure 9.…”

Section: Determination Of Energy Bandgapmentioning

confidence: 99%

Photophysical Characterization of Er3+ doped and Er3+/Nd3+ co-doped Gd(OH)3 Nanocrystals: the Impact of Hexamethylenetetramine utilization for Morphology Engineering

Gbor,

Swart,

Reyes-Rojas

et al. 2024

Preprint

View full text Add to dashboard Cite

This work is a study on the photophysical properties of Er3+ doped and Er3+/ Nd3+ co-doped Gd(OH)3 nanocrystals. It also demonstrated up-conversion luminescence quenching arising from utilization of hexamethylenetetramine (HMTA) for the formation of rod-like morphologies. The synthesis was carried out via the precipitation technique. TG/DTA results show Gd(OH)3 phase is formed and stabilized in the temperature range of 30 to 106o C. Fourier Transform Infrared spectroscopy results indicate the presence of OH functional group, a signature of core OH- of the Gd(OH)3 host as well as the HMTA. X-ray diffraction measurement show hexagonal structure. The average crystallite sizes range between 23 and 35 nm in an increasing trend with increasing Nd3+ concentrations. SEM micrographs showed nanorods only for samples treated with HMTA. The 800 nm transition line was enhanced in the diffuse reflectance measurements with increasing Nd content, suggesting that upconversion emission may be enhanced as well. As Nd3+ ion concentrations increased, the valence band and conduction band edges also showed lateral changes towards more negative values. The Gd(OH)3:Er0.02 nanocrystals (without HMTA) produced the strongest upconversion emission at 560, 660, 758 and 875 nm. The emission at 875 nm had a 10-fold enhancement over the other emission bands. The pair of emissions, 758 and 875 nm, are employed in luminescence thermometry. Thus, the results from this work demonstrated that the synthesized nanocrystals have the potential to be applied in photonic related applications such as photodynamic therapy and luminescence thermometry. No upconversion emission was recorded for Er3+, Nd3+ co-doped Gd(OH)3 nanorods due to prevalence of OH-group from the HMTA used.

show abstract

Synthesis of gadolinium oxide nanocuboids for in vitro bioimaging applications

Cited by 5 publications

References 43 publications

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

Ex vivo interaction study of NaYF₄:Yb,Er nanophosphors with isolated mitochondria

Photophysical Characterization of Er3+ doped and Er3+/Nd3+ co-doped Gd(OH)3 Nanocrystals: the Impact of Hexamethylenetetramine utilization for Morphology Engineering

Contact Info

Product

Resources

About

Synthesis of gadolinium oxide nanocuboids for in vitro bioimaging applications

Cited by 5 publications

References 43 publications

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

Ex vivo interaction study of NaYF4:Yb,Er nanophosphors with isolated mitochondria

Photophysical Characterization of Er3+ doped and Er3+/Nd3+ co-doped Gd(OH)3 Nanocrystals: the Impact of Hexamethylenetetramine utilization for Morphology Engineering

Contact Info

Product

Resources

About

Ex vivo interaction study of NaYF₄:Yb,Er nanophosphors with isolated mitochondria