Synthesis of functionalized polyethylenimine-grafted mesoporous silica spheres and the effect of side arms on lipase immobilization and application

Khoobi, Mehdi; Motevalizadeh, Seyed Farshad; Asadgol, Zahra; Forootanfar, Hamid; Shafiee, Abbas; Faramarzi, Mohammad Ali

doi:10.1016/j.bej.2014.04.009

Cited by 64 publications

(37 citation statements)

References 51 publications

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“…According to the IUPAC nomenclature mesoporous structures are characterized by the pore size from 2 to 50 nm [2]. Particularly, mesoporous silica materials have been extensively studied due to their applications in catalysis [3], sorption and separation processes [4], enzyme immobilization [5], sensor design [6], energy storage [7], antimicrobial carriers [8], and drug delivery [9] among others. Mesoporous silicas have gathered a significant attention in diverse fields of applications for their high chemical stability, large pore volume, high specific surface area and possibility of surface modification [10].…”

Section: Introductionmentioning

confidence: 99%

Aminopropyl-modified mesoporous molecular sieves as efficient adsorbents for removal of auxins

Moritz

Geszke-Moritz

2015

Applied Surface Science

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Section: Introductionmentioning

confidence: 99%

Aminopropyl-modified mesoporous molecular sieves as efficient adsorbents for removal of auxins

Moritz

Geszke-Moritz

2015

Applied Surface Science

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“…The strategy of immobilization has a great impact on the attached enzyme properties-it may improve enzyme activity, stability, selectivity, and specificity [9,10]. In general, disadvantages is immobilization [7,8]. The strategy of immobilization has a great impact on the attached enzyme properties-it may improve enzyme activity, stability, selectivity, and specificity [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the separation of enzyme after reaction is somewhat complicated and needs centrifugation and precipitation. One of the most promising methods to improve the enzyme's disadvantages is immobilization [7,8]. The strategy of immobilization has a great impact on the attached enzyme properties-it may improve enzyme activity, stability, selectivity, and specificity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

Ziegler-Borowska

Chełminiak-Dudkiewicz

Siódmiak

et al. 2017

Catalysts

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This article presents a novel route for crosslinking a polysaccharide and polysaccharide/protein shell coated on magnetic nanoparticles (MNPs) surface via condensation reaction with squaric acid (SqA). The syntheses of four new types of collagen-, chitosan-, and chitosan-collagen coated magnetic nanoparticles as supports for enzyme immobilization have been done. Structure and morphology of prepared new materials were characterized by attenuated total reflectance Fourier-transform infrared (ATR-FTIR), XRD, and TEM analysis. Next, the immobilization of lipase from Candida rugosa was performed on the nanoparticles surface via N-(3-dimethylaminopropyl)-N -ethylcarbodiimide hydrochloride (EDC)/N-hydroxy-succinimide (NHS) mechanism. The best results of lipase activity recovery and specific activities were observed for nanoparticles with polymer shell crosslinked via a novel procedure with squaric acid. The specific activity for lipase immobilized on materials crosslinked with SqA (52 U/mg lipase) was about 2-fold higher than for enzyme immobilized on MNPs with glutaraldehyde addition (26 U/mg lipase). Moreover, a little hyperactivation of lipase immobilized on nanoparticles with SqA was observed (104% and 112%).

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“…The immobilized lipase was applied to the synthesis of ethyl valerate in n-hexane. As a result, the immobilized lipase retained 83% of its initial activity after 12-times reuse and exhibited 79% conversion rate in nhexane [25].…”

Section: Chemical Immobilization On Nanoscale Support Materialsmentioning

confidence: 94%

Recent progress in nanobiocatalysis for enzyme immobilization and its application

Min

Yoo

2014

Biotechnol Bioproc E

146

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Recent advances in nanotechnology have provided various nanoscale materials that can be used as support for enzyme immobilization. Nanobiocatalysis integrating the biocatalyst and nanoscale materials is drawing great attention as innovative technology. Nanobiocatalysis could achieve not only a much higher enzyme loading capacity and a significantly enhanced mass transfer efficiency, but also unbelievable stabilization. In this review, we will present and discuss the recent progress in nanobiocatalysis and its applications in the fields of bioelectronics, bioconversion, and proteomics.

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Synthesis of functionalized polyethylenimine-grafted mesoporous silica spheres and the effect of side arms on lipase immobilization and application

Cited by 64 publications

References 51 publications

Aminopropyl-modified mesoporous molecular sieves as efficient adsorbents for removal of auxins

Aminopropyl-modified mesoporous molecular sieves as efficient adsorbents for removal of auxins

Chitosan–Collagen Coated Magnetic Nanoparticles for Lipase Immobilization—New Type of “Enzyme Friendly” Polymer Shell Crosslinking with Squaric Acid

Recent progress in nanobiocatalysis for enzyme immobilization and its application

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