Abstract.Modified amino acids which can serve as precursors in the synthesis of tritium-labelled peptides were prepared and incorporated into biologically active peptides. Thus, p-iodophenylalanine was incorporated at position 4 of H-Met(0,)-Glu-His-Phe-D-Lys-Phe-OH (Org"2766) and in des-Tyr I-y-endorphin. As a second labelling site, the precursor of L-leucine, L-methallylglycine, was incorporated in both des-Tyrl-y-endorphin and the shorter des-enkephalin-y-endorphin. Finally, the lysine residue in Org 2766 (D-LYS) and des-enkephalin-y-endorphin (L-LYS) was replaced by its analogue 2,6-diamino-4-hexynoic acid. The precursor peptides were synthesized via the fragment condensation approach. With the exception of the methallylglycine-containing des-Tyr l-y-endorphin, in which partial desulfuri~ation of the methionine residue could not be prevented, these peptides could be converted by means of catalytic reduction into their respective parent compounds.