Synthesis of folate- pegylated polyester nanoparticles encapsulating ixabepilone for targeting folate receptor overexpressing breast cancer cells

Sıafaka, Panoraia I.; Betsiou, M.; Tsolou, Avgi; Angelou, E.; Agianian, Bogos; Koffa, Maria; Chaitidou, Sotiria; Karavas, Evangelos; Avgoustakis, Konstantinos; Bikiaris, Dimitrios N.

doi:10.1007/s10856-015-5609-x

Cited by 43 publications

(44 citation statements)

References 35 publications

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“…The presence of amine and carboxylic groups on the capsid surface allows the strategy to attach specific compounds, as ligands, to be recognized by the targeted cells , making these nanovehicles able to deliver therapeutic enzymes. Folate receptors in the tumor cells have been used for nanoparticle targeting …”

Section: Introductionmentioning

confidence: 99%

“…The presence of amine and carboxylic groups on the capsid surface allows the strategy to attach specific compounds, as ligands, to be recognized by the targeted cells [27], making these nanovehicles able to deliver therapeutic enzymes. Folate receptors in the tumor cells have been used for nanoparticle targeting [28,29] The aim of this work is to demonstrate that VLPs bionanoreactors containing cytochrome P450 (CYP) can be specifically targeted to the over-expressed receptors in cancer cells. The functionalization of biocatalytic VLPs with polyethylene glycol (PEG), containing folic acid as receptor ligand, could improve the efficacy of the anti-carcinogenic pro-drug tamoxifen (Scheme 1).…”

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confidence: 99%

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Biocatalytic virus capsid as nanovehicle for enzymatic activation of Tamoxifen in tumor cells

Tapia-Moreno

Juárez-Moreno

González-Davis

et al. 2017

Biotechnology Journal

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Most of the drugs used in chemotherapy should be activated by a transformation catalyzed by cytochrome P450 (CYP) enzymes. In this work, bacteriophage P22 virus-like particles (VLPs) containing CYP activity, immunologically inert and functionalized in order to be recognized by human cervix carcinoma cells and human breast adenocarcinoma cells were designed. The CYP was encapsulated inside the virus capsid obtained from the bacteriophage P22. CYP and coat protein were both heterologously expressed in E. coli. The VLPs with enzymatic activity were covered with polyethylene glycol that was functionalized in its distal end with folic acid in order to be recognized by folate receptors exhibited on tumor cells. The capacity of biocatalytic VLPs to be recognized and internalized into tumor cells is demonstrated. The VLP-treated cells showed enhanced capacity for the transformation of the pro-drug tamoxifen, which resulted in an increase of the cell sensitivity to this oncological drug. In this work, the potential use of biocatalytic VLPs vehicles as a delivery system of medical relevant enzymes is clearly demonstrated. In addition to cancer treatment, this technology also offers an interesting platform as nano-bioreactors for intracellular delivery of enzymatic activity for other diseases originated by the lack of enzymatic activity.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Biocatalytic virus capsid as nanovehicle for enzymatic activation of Tamoxifen in tumor cells

Tapia-Moreno

Juárez-Moreno

González-Davis

et al. 2017

Biotechnology Journal

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“…The mannose moiety allows for targeting of cancer cells that specifically express mannose receptors . Bikiaris and coworkers reported folate‐pegylated polyester nanoparticles using folic acid (FA)‐functionalized PEG‐ b ‐poly(propylene succinate) that are more selective in targeting tumors overexpressing the folate receptor such as those in breast cancer …”

Section: Nonstimuli‐responsive Polyester Nanocarriersmentioning

confidence: 99%

“…61 Bikiaris and coworkers reported folate-pegylated polyester nanoparticles using folic acid (FA)-functionalized PEG-b-poly(propylene succinate) that are more selective in targeting tumors overexpressing the folate receptor such as those in breast cancer. 62 Different architectures have been used for polyester nanocarriers as well including star polymers, worm-like micelles, and polymersomes. Unimolecular star-like block copolymers synthesized from a cyclodextrin core were reported by Wang and coworkers.…”

Section: Nonstimuli-responsive Polyester Nanocarriersmentioning

confidence: 99%

Recent advances in aliphatic polyesters for drug delivery applications

Washington

Kularatne

Karmegam

et al. 2016

WIREs Nanomed Nanobiotechnol

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The use of aliphatic polyesters in drug delivery applications has been a field of significant interest spanning decades. Drug delivery strategies have made abundant use of polyesters in their structures owing to their biocompatibility and biodegradability. The properties afforded from these materials provide many avenues for the tunability of drug delivery systems to suit individual needs of diverse applications. Polyesters can be formed in several different ways, but the most prevalent is the ring-opening polymerization of cyclic esters. When used to form amphiphilic block copolymers, these materials can be utilized to form various drug carriers such as nanoparticles, micelles, and polymersomes. These drug delivery systems can be tailored through the addition of targeting moieties and the addition of stimuli-responsive groups into the polymer chains. There are also different types of polyesters that can be used to modify the degradation rates or mechanical properties. Here, we discuss the reasons that polyesters have become so popular, the current research focuses, and what the future holds for these materials in drug delivery applications. WIREs Nanomed Nanobiotechnol 2017, 9:e1446. doi: 10.1002/wnan.1446 For further resources related to this article, please visit the WIREs website.

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“…There are three isoforms of FR (FRα, FRβ and FRγ) and type α is the major isoform mediating folate transport, and is overexpressed on the surface of various types of tumor, including pancreatic, prostate, lung, head and neck, breast and ovarian cancer, and mesothelioma (11–19). Based on its limited expression and restricted distribution pattern in normal tissue, FRα is the most widely studied member of the FR family, and various strategies for targeting FR, which is overexpressed in cancer, have been developed (20,21). …”

Section: Introductionmentioning

confidence: 99%

Folate receptor-targeted ultrasonic PFOB nanoparticles: Synthesis, characterization and application in tumor-targeted imaging

Liu

Zhang

et al. 2017

International Journal of Molecular Medicine

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Abstract. In this study, we aimed to determine an effective strategy for the synthesis of folate receptor (FR) targetednanoparticles (FRNPs). The nanoparticles used as ultrasound contrast agents (UCAs) were composed of a liquid core of perfluorooctyl bromide (PFOB) liposome and a targeted shell chemically conjugated with folic acid (FA) and polyethylene glycol (PEG). This was done in order to avoid recognition and clearance by the mononuclear phagocyte system [also known as the reticuloendothelial system (RES)] and enhance the targeting capability of the nanoparticles to tumors overexpressing folate receptor (FR). The FRNPs exhibited an average particle size of 301±10.8 nm and surface potential of 39.1±0.43 mV. Subsequently, in vitro, FRNPs labeled with FITC fluorescence dye were visibly uptaken into the cytoplasm of FR-overexpressing cancer cells (Bel7402 and SW620 cells), whereas the A549 cells expressing relatively low levels of FR just bound with few FRNPs. These results demonstrated that FRNPs have a high affinity to FR-overexpressing cancer cells. Additionally, in in vivo experiments, FRNPs achieved a greater enhancement of tumor ultrasound imaging and a longer enhancement time in FR-overexpressing tumors and the Cy7-labeled FRNPs exhibited a relatively high tumor-targeted distribution in FR-overexpressing tumors. Targeted ultrasound and fluorescence imaging revealed that FRNPs have the ability to target FR-overexpressing tumors and ex vivo fluorescence imaging was then used to further verify and confirm the presence of FRNPs in tumor tissues with histological analysis of the tumor slices. On the whole, our data demonstrate that the FRNPs may prove to be a promising candidate for the early diagnosis for FR-overexpressing tumors at the molecular and cellular levels.

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Synthesis of folate- pegylated polyester nanoparticles encapsulating ixabepilone for targeting folate receptor overexpressing breast cancer cells

Cited by 43 publications

References 35 publications

Biocatalytic virus capsid as nanovehicle for enzymatic activation of Tamoxifen in tumor cells

Biocatalytic virus capsid as nanovehicle for enzymatic activation of Tamoxifen in tumor cells

Recent advances in aliphatic polyesters for drug delivery applications

Folate receptor-targeted ultrasonic PFOB nanoparticles: Synthesis, characterization and application in tumor-targeted imaging

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