Synthesis of Fluorescent BODIPY‐Labeled Analogue of Miltefosine for Staining of Acanthamoeba.

Abstract

Amoebae pose a potential health risk as pathogenic agents i. e. in the central nervous system or the cornea, early diagnostics are important to implement rapid treatments and reduce impact on human health. Thus, development of selective fluorescent tags for Amoebae is an important issue. Analogues of Miltefosine, a drug effective against various strains of freeliving amoebae, have been synthesized. They are derivatives of the 11-aminoundecylphosphocholine. Via a peptide bond, a strong emitting BODIPY was embedded to the structure. This compound (1) was used to selectively stain for Acanthamoeba castellanii. In order to test the effect of the insertion of the amide function in the alkylbackbone of this modified Miltefosine, a model molecule (2) was prepared by replacing the dye by the anisidyl moiety. Anti-amoebic activity was evaluated for both.

Synthesis, Optical Properties, Preliminary Antimycobacterial Evaluation and Docking Studies of Trifluoroacetylated 3‐Pyrrolyl Boron‐Dipyrromethene

“…Boron‐dipyrromethene (BODIPY) class of compounds was first described in 1968 [1] . Since then, BODIPY dyes have become one of the most popular fluorophores and are used as emissive dyes, labels, probes, and sensors for diverse applications [2–11] . The reason is strongly absorbing small molecules with sharp absorption and emission bands, and high fluorescence quantum yields.…”

“…[1] Since then, BODIPY dyes have become one of the most popular fluorophores and are used as emissive dyes, labels, probes, and sensors for diverse applications. [2][3][4][5][6][7][8][9][10][11] The reason is strongly absorbing small molecules with sharp absorption and emission bands, and high fluorescence quantum yields. They are reasonably stable at physiological conditions.…”