Synthesis of fibroin in the cultured glands of Nephila clavipes

Candelas, Graciela C.; López, Félix de la Paz

doi:10.1016/0305-0491(83)90242-0

Cited by 17 publications

(16 citation statements)

References 7 publications

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“…We also show the effects of various tRNA complements on the process. Through the labeling kinetics strategies used in our previous work on the intact glands (Candelas et al 1983), the association of the smaller peptides to the process of fibroin synthesis is confirmed.…”

Section: Introductionsupporting

confidence: 55%

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Features of the cell-free translation of a spider fibroin mRNA

Candelas

Ortiz²,

Ortiz³

1989

Biochem. Cell Biol.

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The massive production of fibroin by the large ampullate glands of the spider, Nephila clavipes, serves as a model system in which to study the synthesis and control of a large secretory protein. Their tissue-specific product, fibroin, produced during the entire adult life of the female, is approximately 320 kilodaltons, and rich in glycine and alanine. Highly purified fibroin mRNA from the glands has been translated in a rabbit reticulocyte cell-free system with variable supplements. The translational products analyzed by SDS-PAGE display two features, tRNA modulation and discontinuous pauses during elongation. tRNA complements exert their effects both in the translational efficiency and in the size of the peptides generated. The pauses observed during translation generate subsets of smaller discrete peptides, visualized in the gels as ladders of variable relative intensities, appearing exclusively and concomitantly with the fibroin. Definitive linkage between the discrete peptides and fibroin synthesis process has been established by their selective labeling with specific radioactive amino acids.

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Section: Introductionsupporting

confidence: 55%

“…These are evidenced as subsets of discrete smaller peptides, generated only during the gland's active fibroin production (Candelas and Cintron 1981;Candelas and Lopez 1983;Candelas et al 1983). The selective labeling of the peptides by radioactive amino acids confirmed their identity as nascent fibroin chains (Candelas el al.…”

Section: Introductionmentioning

confidence: 84%

Features of the cell-free translation of a spider fibroin mRNA

Candelas

Ortiz²,

Ortiz³

1989

Biochem. Cell Biol.

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“…Moreover, the secretory silk is produced in a form which is ready for secretion, and undergoes no further concentration. Candelas and Cintron (1981) and Candelas and Lopez (1983) also reported that the secretory product of the large ampullate glands of Nephih chvpes migrates as one homogeneous band in denaturing electrophoresis, and that the luminal contents comprise the liquid silk.…”

Section: Discussionmentioning

confidence: 99%

Fine structure of the glandular epithelium during secretory silk production in the black widow spiderlatrodectus mactans

Moon

Tillinghast

2002

Korean Journal of Biological Sciences

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“…According to Candelas and Cintron (1981) and Candelas and Lopez (1983), secretory product of the large ampullate glands of Nephila clavipes migrates as one homogeneous band in denaturing electrophoresis, and the luminal contents comprise the liquid silk. It is clear that secretory products of the ampullate silk glands in this spider are accumulated in luminal cytoplasm of the glandular epithelium as a form of electron dense granules.…”

Section: Discussionmentioning

confidence: 99%