Synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications

Ogushi, Yuko; Sakai, Shinji; Kawakami, Koei

doi:10.1263/jbb.104.30

Cited by 148 publications

(92 citation statements)

References 12 publications

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“…However, even if no phenolic side chain is present in the polymer, it may be introduced chemically. In that respect, tyramine has gained some popularity e.g., for the crosslinking of carboxymethyl cellulose [154], alginate [155], hyaluronic acid [156], dextran [157] or artificial polymers such as Tetronic [158].…”

Section: Polymer Modificationmentioning

confidence: 99%

Enzyme Initiated Radical Polymerizations

2012

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Section: Polymer Modificationmentioning

confidence: 99%

Enzyme Initiated Radical Polymerizations

2012

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“…CMC is a biocompatible, low-cost, FDA-approved material that is commercially available in high purity forms, making this polymer a highly attractive option for biomedical applications (Ogushi et al, 2007). Photocrosslinked CMC has been recently shown to produce stable hydrogels, support NP cell viability, and promote phenotypic matrix deposition capable of maintaining initial mechanical properties in vitro (Reza and Nicoll, 2009).…”

Section: Introductionmentioning

confidence: 99%

Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

Reza

Nicoll

2009

Biotech & Bioengineering

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Degeneration of the nucleus pulposus (NP) has been implicated as a major cause of low back pain. Tissue engineering strategies may provide a viable NP replacement therapy; however, culture conditions must be optimized to promote functional tissue development. In this study, a standard serum-containing medium formulation was compared to a chemically defined, serum-free medium to determine the effect on matrix elaboration and functional properties of NP cell-laden carboxymethylcellulose (CMC) hydrogels. Additionally, both media were further supplemented with transforming growth factor-beta 3 (TGF-beta(3)). Glycosaminoglycan (GAG) content increased in both TGF-beta(3)-treated groups and was highest for treated, serum-free constructs (9.46 +/- 1.51 microg GAG/mg wet weight), while there were no quantifiable GAGs in untreated serum-containing samples. Histology revealed uniform, interterritorial staining for chondroitin sulfate proteoglycan throughout the treated, serum-free constructs. Type II collagen content was greater in both serum-free groups and highest in treated, serum-free constructs. The equilibrium Young's modulus was highest in serum-free samples supplemented with TGF-beta(3) (18.54 +/- 1.92 kPa), and the equilibrium weight swelling ratio of these constructs approached that of the native NP tissue (22.19 +/- 0.46 vs. 19.94 +/- 3.09, respectively). Taken together, these results demonstrate enhanced functional matrix development by NP cells when cultured in CMC hydrogels maintained in serum-free, TGF-beta(3) supplemented medium, indicating the importance of medium formulation in NP construct development.

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“…Divalent cations are necessary for maintaining the homoeostasis of mammalian cells, thus, a shorter exposure time to the solution containing no divalent cations is preferable. In the present study, we investigate the feasibility of carboxymethylcellulose derivatives having phenol moieties (CMC-Ph) (Ogushi et al 2007) as materials for subsieve-size capsules prepared via the processes developed for preparing subsieve-size Alg-Ph capsules (Sakai et al 2007a, b). Aqueous CMC solution does not gel by contacting with divalent cations in the medium and physiological buffers, thus, solutions containing divalent cations can be used for dissolving CMC.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of carboxymethylcellulose with phenol moieties as a material for mammalian cell-enclosing subsieve-size capsules

et al. 2008

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Mammalian cell-enclosing capsules have been investigated as devices for bioproduction, cell therapy and stem cell research. In this study, carboxymethylcellulose (CMC) with phenol moieties (CMC-Ph), synthesized through the conjugation reaction of CMC and tyramine, was investigated as a material for these types of devices. Subsieve-size capsules of less than 100 lm in diameter were prepared by extruding aqueous CMC-Ph solution into co-flowing liquid paraffin containing H 2 O 2 . The capsule diameter was controlled between 60-220 lm by changing the flow rate of liquid paraffin. There was no harmful effect specific for CMC-Ph on mammalian cells enclosed in capsules. Feline kidney cells enclosed in subsieve-size CMC-Ph capsules exhibited 87.0 ± 4.5% viability. In addition, the enclosed cells continued to grow over 13 days of study. These results demonstrated the feasibility of CMC-Ph as a material for subsieve-size cell-enclosing capsules prepared via the droplet breakup technique in a co-flowing water-immiscible fluid.

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Synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications

Cited by 148 publications

References 12 publications

Enzyme Initiated Radical Polymerizations

Enzyme Initiated Radical Polymerizations

Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

Feasibility of carboxymethylcellulose with phenol moieties as a material for mammalian cell-enclosing subsieve-size capsules

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Synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications

Cited by 148 publications

References 12 publications

Enzyme Initiated Radical Polymerizations

Enzyme Initiated Radical Polymerizations

Serum‐free, chemically defined medium with TGF‐β3 enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

Feasibility of carboxymethylcellulose with phenol moieties as a material for mammalian cell-enclosing subsieve-size capsules

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Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs