Synthesis of DNA-templated copper nanoparticles with enhanced fluorescence stability for cellular imaging

Kim, Seokjoon; Kim, Jung Ho; Kwon, Woo Young; Hwang, Sun Hee; Seok, Byung; Kim, Ji Min; Oh, Seung Soo; Park, Ki Soo

doi:10.1007/s00604-019-3620-5

Cited by 29 publications

(17 citation statements)

References 19 publications

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“…The functionalization of nanoparticles with macromolecular structures like DNA and RNA is quickly gathering interest as a tool for sensing applications and drug-delivery in medicine and biology. − When functionalized, the nanoparticles can travel within the biological system and be transported to a target region. Upon irradiation, the functionalized nanoparticles boost the optical response of the surrounding molecules.…”

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confidence: 99%

Photoprotecting Uracil by Coupling with Lossy Nanocavities

Felicetti

Fregoni

Schnappinger

et al. 2020

J. Phys. Chem. Lett.

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We analyze how the photorelaxation dynamics of a molecule can be controlled by modifying its electromagnetic environment using a nanocavity mode. In particular, we consider the photorelaxation of the RNA nucleobase uracil, which is the natural mechanism to prevent photodamage. In our theoretical work, we identify the operative conditions in which strong coupling with the cavity mode can open an efficient photoprotective channel, resulting in a relaxation dynamics twice as fast as the natural one. We rely on a state-of-the-art chemically detailed molecular model and a non-Hermitian Hamiltonian propagation approach to perform full-quantum simulations of the system dissipative dynamics. By focusing on the photon decay, our analysis unveils the active role played by cavity-induced dissipative processes in modifying chemical reaction rates, in the context of molecular polaritonics. Remarkably, we find that the photorelaxation efficiency is maximized when an optimal trade-off between light–matter coupling strength and photon decay rate is satisfied. This result is in contrast with the common intuition that increasing the quality factor of nanocavities and plasmonic devices improves their performance. Finally, we use a detailed model of a metal nanoparticle to show that the speedup of the uracil relaxation could be observed via coupling with a nanosphere pseudomode, without requiring the implementation of complex nanophotonic structures.

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confidence: 99%

Photoprotecting Uracil by Coupling with Lossy Nanocavities

Felicetti

Fregoni

Schnappinger

et al. 2020

J. Phys. Chem. Lett.

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“…The stability of synthesized copper nanoparticles is always of great concern as these can be easily oxidized on contact with air; therefore, these reactions are always performed under an inert atmosphere by purging the reaction vessel with nitrogen or argon gas. Protecting layers of organic and inorganic materials are usually employed to impart stability to nanoparticles and thus to prevent surface oxidation of copper ( Begletsova et al., 2018 ; Kim et al., 2019 ; Suárez-Cerda et al., 2017 ). Protective agents like toluene, dodecanethiol, triethylamine, carbon, silicon, polyethylene glycols, polyacrylic acid, sodium dodecyl benzene sulfonate/sodium dodecyl sulfate, lauric acid etc.…”

Section: Synthesis Of Nzvcmentioning

confidence: 99%

Nanoscale zerovalent copper (nZVC) catalyzed environmental remediation of organic and inorganic contaminants: A review

Kumar

Kaur²,

Brar³

et al. 2022

Heliyon

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“…Representative examples of these types of nanomaterial are gold, silver, copper, and platinum NCs that are synthesized via their interactions with proteins or nucleic acids [ 4 ]. AuNCs are generally formed on a protein template, while AgNCs and CuNCs are formed on a nucleic acid template [ 5 , 6 , 7 ]. Bovine serum albumin (BSA) was the first protein used to produce AuNCs, which have been widely employed for cellular imaging and biomolecular sensing [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications

Lee

Seok

Kim

et al. 2021

IJMS

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In recent years, fluorescent metal nanoclusters have been used to develop bioimaging and sensing technology. Notably, protein-templated fluorescent gold nanoclusters (AuNCs) are attracting interest due to their excellent fluorescence properties and biocompatibility. Herein, we used an exosome template to synthesize AuNCs in an eco-friendly manner that required neither harsh conditions nor toxic chemicals. Specifically, we used a neutral (pH 7) and alkaline (pH 11.5) pH to synthesize two different exosome-based AuNCs (exo-AuNCs) with independent blue and red emission. Using field-emission scanning electron microscopy, energy dispersive X-ray microanalysis, nanoparticle tracking analysis, and X-ray photoelectron spectroscopy, we demonstrated that AuNCs were successfully formed in the exosomes. Red-emitting exo-AuNCs were found to have a larger Stokes shift and a stronger fluorescence intensity than the blue-emitting exo-AuNCs. Both exo-AuNCs were compatible with MCF-7 (human breast cancer), HeLa (human cervical cancer), and HT29 (human colon cancer) cells, although blue-emitting exo-AuNCs were cytotoxic at high concentrations (≥5 mg/mL). Red-emitting exo-AuNCs successfully stained the nucleus and were compatible with membrane-staining dyes. This is the first study to use exosomes to synthesize fluorescent nanomaterials for cellular imaging applications. As exosomes are naturally produced via secretion from almost all types of cell, the proposed method could serve as a strategy for low-cost production of versatile nanomaterials.

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Synthesis of DNA-templated copper nanoparticles with enhanced fluorescence stability for cellular imaging

Cited by 29 publications

References 19 publications

Photoprotecting Uracil by Coupling with Lossy Nanocavities

Photoprotecting Uracil by Coupling with Lossy Nanocavities

Nanoscale zerovalent copper (nZVC) catalyzed environmental remediation of organic and inorganic contaminants: A review

Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications

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