Synthesis of DNA-encapsulated silica elaborated by sol–gel routes

Kapusuz, Derya; Durucan, Caner

doi:10.1557/jmr.2012.309

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…Several cold and dry storage strategies, eventually combined and improved by storage additives, , are used to store/transport DNA. Alternative solutions, such as DNA encapsulation into porous silica matrix , or within layered metal hydroxide, , have also been proposed.…”

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confidence: 99%

Magnetically Recoverable, Thermostable, Hydrophobic DNA/Silica Encapsulates and Their Application as Invisible Oil Tags

et al. 2014

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A method to encapsulate DNA in heat-resistant and inert magnetic particles was developed. An inexpensive synthesis technique based on co-precipitation was utilized to produce Fe2O3 nanoparticles, which were further functionalized with ammonium groups. DNA was adsorbed on this magnetic support, and the DNA/magnet nanocluster was surface coated with a dense silica layer by sol-gel chemistry. The materials were further surface modified with hexyltrimethoxysilane to achieve particle dispersibility in hydrophobic liquids. The hydrodynamic particle sizes were evaluated by analytical disc centrifugation, and the magnetic properties were investigated by vibrating sample magnetometry. The obtained nanoengineered encapsulates showed good dispersion abilities in various nonaqueous fluids and did not affect the optical properties of the hydrophobic dispersant when present at concentrations lower than 10(3) μg/L. Upon magnetic separation and particle dissolution, the DNA could be recovered unharmed and was analyzed by quantitative real-time PCR and Sanger sequencing. DNA encapsulated within the magnetic particles was stable for 2 years in decalin at room temperature, and the stability was further tested at elevated temperatures. The new magnetic DNA/silica encapsulates were utilized to developed a low-cost platform for the tracing/tagging of oils and oil-derived products, requiring 1 μg/L=1 ppb levels of the taggant and allowing quantification of taggant concentration on a logarithmic scale. The procedure was tested for the barcoding of a fuel (gasoline), a cosmetic oil (bergamot oil), and a food grade oil (extra virgin olive oil), being able to verify the authenticity of the products.

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confidence: 99%

Magnetically Recoverable, Thermostable, Hydrophobic DNA/Silica Encapsulates and Their Application as Invisible Oil Tags

et al. 2014

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“…32 In a previous study, EtBr molecules were shown to intercalate into ds-DNA molecules encapsulated in an amorphous silica network. 26 So, accumulation of EtBr onto E-silica DNA , as evidenced by optical analyses, indicates presence of preferential sites for EtBr accommodation in DNA even after encapsulation state. Hence, the native base-paired, helix conformation of DNA seems to be preserved in the gel after sol-gel transformation.…”

Section: Resultsmentioning

confidence: 93%

“…As EtBr is a planar molecule and can intercalate only into ds-DNA, it was used as a marker to show that ds-DNA molecules were trapped in silica and the procedure was described elsewhere. 26 Basically in a typical test, an EtBr solution was prepared by dissolving EtBr powders in DI-water (10 µg/mL). Then, E-silica DNA and E-silica dAMP gel powders (35 mg) were immersed into EtBr solution (3.5 mL) for 3 h. After 3 h, the powders were separated from the solution by centrifugation (10,000 r/min, 5 min) and the supernatants were analyzed by UV-Vis spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…In fact, a previous work describes effective encapsulation of ds-DNA in silica by a sol-gel route. 26 It was shown that, the alkaline DNA-buffer hydrolysis solution leads to pH increase and to faster gelation. Therefore, condensation of silica clusters enhances with DNA addition, culminating in an open, mesoporous structure at a final pH around 7–8.…”

Section: Introductionmentioning

confidence: 99%

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Exploring encapsulation mechanism of DNA and mononucleotides in sol-gel derived silica

Kapusuz

Durucan

2017

J Biomater Appl

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The encapsulation mechanism of DNA in sol-gel derived silica has been explored in order to elucidate the effect of DNA conformation on encapsulation and to identify the nature of chemical/physical interaction of DNA with silica during and after sol-gel transition. In this respect, double stranded DNA and dAMP (2'-deoxyadenosine 5'-monophosphate) were encapsulated in silica using an alkoxide-based sol-gel route. Biomolecule-encapsulating gels have been characterized using UV-Vis, Si NMR, FTIR spectroscopy and gas adsorption (BET) to investigate chemical interactions of biomolecules with the porous silica network and to examine the extent of sol-gel reactions upon encapsulation. Ethidium bromide intercalation and leach out tests showed that helix conformation of DNA was preserved after encapsulation. For both biomolecules, high water-to-alkoxide ratio promoted water-producing condensation and prevented alcoholic denaturation. NMR and FTIR analyses confirmed high hydraulic reactivity (water adsorption) for more silanol groups-containing DNA and dAMP encapsulated gels than plain silica gel. No chemical binding/interaction occurred between biomolecules and silica network. DNA and dAMP encapsulated silica gelled faster than plain silica due to basic nature of DNA or dAMP containing buffer solutions. DNA was not released from silica gels to aqueous environment up to 9 days. The chemical association between DNA/dAMP and silica host was through phosphate groups and molecular water attached to silanols, acting as a barrier around biomolecules. The helix morphology was found not to be essential for such interaction. BET analyses showed that interconnected, inkbottle-shaped mesoporous silica network was condensed around DNA and dAMP molecules.

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“…Therefore, the TMG precursor might favor the dissociation reaction more than TMI. Finally, the distorted peak positioned between −85 and −120 ppm is clearly assignable to silanol groups and bridged silicon of Q 3+4 (O 3 -Si-OH, O 4 -Si) [100,102].…”

Section: Determination Of Ligand Implementationmentioning

confidence: 99%

Synthesis of High Surface Area—Group 13—Metal Oxides via Atomic Layer Deposition on Mesoporous Silica

et al. 2022

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The atomic layer deposition of gallium and indium oxide was investigated on mesoporous silica powder and compared to the related aluminum oxide process. The respective oxide (GaOx, InOx) was deposited using sequential dosing of trimethylgallium or trimethylindium and water at 150 °C. In-situ thermogravimetry provided direct insight into the growth rates and deposition behavior. The highly amorphous and well-dispersed nature of the oxides was shown by XRD and STEM EDX-mappings. N2 sorption analysis revealed that both ALD processes resulted in high specific surface areas while maintaining the pore structure. The stoichiometry of GaOx and InOx was suggested by thermogravimetry and confirmed by XPS. FTIR and solid-state NMR were conducted to investigate the ligand deposition behavior and thermogravimetric data helped estimate the layer thicknesses. Finally, this study provides a deeper understanding of ALD on powder substrates and enables the precise synthesis of high surface area metal oxides for catalytic applications.

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Synthesis of DNA-encapsulated silica elaborated by sol–gel routes

Abstract: Abstract

Cited by 9 publications

References 44 publications

Magnetically Recoverable, Thermostable, Hydrophobic DNA/Silica Encapsulates and Their Application as Invisible Oil Tags

Magnetically Recoverable, Thermostable, Hydrophobic DNA/Silica Encapsulates and Their Application as Invisible Oil Tags

Exploring encapsulation mechanism of DNA and mononucleotides in sol-gel derived silica

Synthesis of High Surface Area—Group 13—Metal Oxides via Atomic Layer Deposition on Mesoporous Silica

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