Synthesis of DNA and of Hydrolytic Enzymes in Cotyledons of Germinating Seeds of Agrostemma githago L.1)1)Herrn Prof. Dr. H. Borriss zum 70. Geburtstag in Verehrung gewidmet.

Hecker, Michael; Bernhardt, D.; Köhler, Karl-Hermann; Schmerder, B.; Dunger, H.; Knösche, R.

doi:10.1016/s0015-3796(17)30606-6

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1982

1986

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Cited by 10 publications

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Biochemistry of the Multiple Forms of Glycosidases in Plants

1984

Advances in Enzymology - And Related Areas of Molecular Biology

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Biochemistry of the Multiple Forms of Glycosidases in Plants

1984

Advances in Enzymology - And Related Areas of Molecular Biology

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Synthesis of DNA in excised watermelon cotyledons grown in water and benzyladenine

Galli¹

1984

Planta

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Excised watermelon cotyledons were grown in water and benzyladenine, which greatly promotes growth, breakdown of reserves and development of organelles. In order to investigate the involvement of DNA synthesis in these benzyladenine-induced effects, [(3)H]thymidine was applied continuously (for 3 d) or administered briefly (5 h) to excised cotyledons at various stages of development. Autoradiographic analysis of squashed and sectioned cotyledons showed that both the cytoplasm (mainly in the region of the plastids) and most of the nuclei were labelled. Both types of labelling were promoted by benzyladenine treatment. The highest percentage of labelled nuclei was found in the early stages of growth (first day after excision of cotyledons), long before the burst of enzymatic activities involved in the germination processes. The possible meaning of the increase of nuclear DNA, apart from the normal replicative synthesis preceding cell division, is discussed.

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Amylolytic activity in germinated Agrostemma githago L. seeds

Klerk

Willekens

Kok

et al. 1986

Planta

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The perisperm of seeds of Agrostemma githago contains starch reserves which constitute 40% of the dry weight of the mature seed. These starch reserves were mostly broken down between 48 and 96 h after initiation of imbibition. (Germination occurred after 24 h.) The mode of starch degradation showed close parallels with the breakdown of the starchy endosperm in cereals. Thus, between 24 and 96 h the cotyledons secreted α-amylase (EC 3.2.1.1) whereas other degradative enzymes in the perisperm, β-amylase (EC 3.2.1.2) and maltase (EC 3.2.1.20), appeared to originate in the perisperm itself. Cotyledons secreted similar levels of α-amylase in the presence and absence of exogenous starch, indicating that secretion is an internal developmental event of the embryo. By isoelectric focussing the secreted α-amylase was separated into two isoenzymes. In the cotyledons, several other starch-degrading isoenzymes were present but were not secreted.

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Synthesis of DNA and of Hydrolytic Enzymes in Cotyledons of Germinating Seeds of Agrostemma githago L.1)1)Herrn Prof. Dr. H. Borriss zum 70. Geburtstag in Verehrung gewidmet.

Cited by 10 publications

References 10 publications

Biochemistry of the Multiple Forms of Glycosidases in Plants

Biochemistry of the Multiple Forms of Glycosidases in Plants

Synthesis of DNA in excised watermelon cotyledons grown in water and benzyladenine

Amylolytic activity in germinated Agrostemma githago L. seeds

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