Synthesis of Cyanohydrins Using Hydroxynitrile Lyases

Avi, Manuela; Griengl, Herfried; Roberge, Chris; Fleitz, Fred J.; Devine, Paul N.; Langermann, Jan von; Mell, Annett; Paetzold, Eckhard; Kragl, Udo; Asano, Yasuhisa

doi:10.1002/9780470748589.ch8

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“…Me HNL on the other hand has its optimal reaction parameters at pH 5.5 and 60°C ( Andexer et al, 2009 ; Guterl et al, 2009 ) ( Figure 8 ). However, HNLs are generally used significantly below these parameters, e.g., in a pH range of 3–5 and temperatures below 10°C, which is required to suppress product racemization and decomposition of the respective cyanohydrin products ( Von Langermann et al, 2008 ; Avi et al, 2009 ).…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Enzyme Compartments in UV-Cured Polyurethane-Based Materials and Their Application in Enzymatic Reactions

Uhrich

Langermann

2017

Front. Microbiol.

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The preparation and characterization of UV-cured polyurethane-based materials for the mild inclusion immobilization of enzymes was investigated. Full curing of the polymer precursor/enzyme solution mixture was realized by a short irradiation with UV-light at ambient temperatures. The included aqueous enzyme solution remains highly dispersed in the polymer material with an even size distribution throughout the polymer material. The presented concept provides stable enzyme compartments which were applied for an alcohol dehydrogenase-catalyzed reduction reaction in organic solvents. Cofactor regeneration was achieved by a substrate-coupled approach via 2-propanol or an enzyme-coupled approach by a glucose dehydrogenase. This reaction concept can also be used for a simultaneous application of contrary biocatalytic reaction conditions within an enzymatic cascade reaction. Independent polymer-based reaction compartments were provided for two incompatible enzymatic reaction systems (alcohol dehydrogenase and hydroxynitrile lyase), while the relevant reactants diffuse between the applied compartments.

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