Synthesis of Collagen Is Dysregulated in Cultured Fibroblasts Derived From Skin of Subjects With Varicose Veins as It Is in Venous Smooth Muscle Cells

Abstract

Background-The dilatation and tortuosity observed in varicose veins provide evidence for progressive venous wall remodeling associated with abnormalities of smooth muscle cells and extracellular matrix. The present study was designed to examine if the phenotypic modulations observed in the venous smooth muscle cells of patients with varicose veins were also present in their dermal fibroblasts. Methods and Results-Collagen type I (collagen I), type III (collagen III), and type V (collagen V) were compared in dermal fibroblasts derived from the skin of control subjects and patients with varicose veins. The synthesis of collagen I, the release of its metabolites, and the expression of its mRNA were increased in fibroblasts from patients with varicose veins, whereas the synthesis of collagen III was decreased but not correlated with a decrease in mRNA expression and in metabolite release. Matrix metalloproteinases (MMP1, 2, 7, 8, 9, and 13) and their inhibitors (TIMP1 and 2) were quantified in both cell types; only the production of proMMP2 was increased in cells derived from patients with varicose veins. Conclusions-These

The inflammatory reaction during chronic venous disease of lower limbs.

“…In varicose veins alterations of elastic fibers structure are present and they are separated from smooth muscle fibers [17]. A deficiency of type III collagen was observed in CVD patients [18].…”

Pattern of Presentation of Chronic Venous Insufficiency in CVI Clinic of CMCH

“…Chronic venous insufficiency may affect globally 25-50% of adult population and its costs both personal to individual and economic to society are well documented [12][13][14][15][16][17][18][19][20] 26 studies, which shows association between varicose vein and obesity or overweight, is more marked in female than in male. In this study shows among 85 cases of CVI (chronic venous insufficiency) studied 04 (4.65%) were in C1, 54(62.79%) were in C2, 03(3.49%) were in C3, 11(12.79%) were in C4, 6 (6.98%) were in C5 and 8(9.30%) were in C6 according to clinical CEAP classification.…”

Pri̇mer Kroni̇k Venöz Yetmezli̇kli̇ Hastalarda Ti̇roi̇d Hormon Durumu

“…Tüm veriler için P<0,05 anlamlı kabul edildi. (4)(5)(6). Ancak bildiğimiz kadarıyla KVY'nin tiroid hastalıkları ya da bu hastalardaki tiroid hormon seviyeleri daha önce klinik çalışmalarla araştırılmamıştır.…”

“…Altta yatan fizyopatolojinin venöz geri dönüşün azalması ve doluş basıncının artması sonucu gelişen venöz hipertansiyonun damar duvarın-da gerilime neden olduğu ve bunun variköz genişleme ve fonksiyon kaybına yol açtığı düşü-nülmektedir (4). Histolojik çalışmalarda damar duvarındaki endotel ve düz kasların hücre aktivitelerinde remodeling aşamasında üretilen ekstrasellüler matrix proteinlerinin bileşenle-rinde farklılık gösterilmiştir (5). Kollajen-elastin dengesinin kaybolduğunu ve tip 1 ve tip 3 kollajenin arttığı ayrıca, matrikste metalloproteinazlardan 2 ve 9'un aktivitesinin arttığı tespit edilmiştir (6).…”