Synthesis of Chlorotoxin by Native Chemical Ligation

Abstract

Complexes of nickel(II), cobalt(II), copper(II), zinc(II), and manganese(II) are derived from the ligand, (N-(3-methoxyphenyl)-2-[(2E)-3-phenylprop-2-enoyl]hydrazinecarboxamide [MPH] and structurally characterized by various physicochemical and spectral tools such as FTIR, UV-Visible, 1 H NMR, LC-Mass, P-XRD and TGA-DTC. These studies showed that the ligand coordinated to the 3d metal ions in bidentate manner. X-ray diffraction studies indicates that the Ni(II), Cu(II) and Zn(II) complexes are crystalline in nature. Degradation mechanisms, thermodynamic and kinetic parameters of the synthesized metal complexes have been evaluated. The prepared ligand and complexes were evaluated for in-vitro antioxidant DPPH assay, in which metal complexes showed excellent activity. The molecular docking analysis by using human antioxidant enzyme DTT (PDB: 3MNG) have also been evaluated. The ligand and their metal complexes were screened for their antimicrobial activities against different pathogenic bacterial and fungal species.