Opening of the extra ring in pheophorbide a methyl ester by the action of amines differing in the number, size, and structure of substituents on the nitrogen atom was studied with a view to synthesize chlorine e 6 13-carboxamides. Main factors restricting one-step preparation of 13-carboxamides with a desired substituent were revealed. The obtained experimental data can be used as a basis for predicting the possibility for onestep preparative synthesis of particular chlorin e 6 13-carboxamide via reaction with one or another amine.Natural chlorins are known as starting compounds for the synthesis of biologically active substances, such as diagnostic and antitumor agents for oncology, wound healing preparations, etc. [1][2][3][4][5][6][7]. Development of efficient procedures for the introduction of additional substituents at the periphery of chlorin macroring attracts strong interest from the viewpoint of analysis of structure-biological activity relationships and synthesis of new biologically active compounds. In the preparation of medical agents, of particular importance are experimentally simple reactions leading to formation of large series of structurally related compounds. The above requirements could be met by opening of the extra ring in pheophorbide a methyl ester (I) by the action of amines. These reactions could give rise to the corresponding chlorin e 6 13-carboxamides (Schemes 1, 2) [8][9][10][11][12][13][14][15][16][17][18]. The products of such transformations contain various fragments attached to the nitrogen atom at the periphery of the chlorin macroring; therefore, opening of the extra ring in pheophorbide a methyl ester (I) by the action of amines is successfully used to synthesize in one step chlorine e 6 13-carboxamides having various substituents at the periphery.The available published data do not allow us to denote the scope of application of the above reaction in the preparative chemistry of chlorophylls and their derivatives. Amines used previously had fairly simple structure and spatially accessible nitrogen atom linked to aliphatic substituents, so that the effect of substituent on the nitrogen atom could not be estimated. Cleavage of the extra ring in I by the action of amines was reported to occur only at room temperature (reaction time 1-48 h) in chloroform, methylene chloride, or THF; therefore, we were unable to reveal the effect of reaction condition on the basis of published data. From the mechanistic viewpoint, opening of the extra ring in pheophorbide a methyl ester (I) and its analogs by the action of amines is nucleophilic substitution at the carbonyl carbon atom in position 13 1 (Scheme 2). This process, like all analogous reactions of carbonyl compounds, is influenced by the partial negative charge on the unshared electron pair on the nitrogen atom, as well as by the size, structure, and number of substituents thereon, which determine spatial accessibility of the unshared electron pair and formation of tetrahedral (sp 3 ) intermediate [16]. Published data on the reaction me...