Synthesis of Chitosan-Based Gold Nanoparticles: Antimicrobial and Wound-Healing Activities

Abstract

The global spread of multidrug-resistant bacteria has become a significant hazard to public health, and more effective antibacterial agents are required. Therefore, this study describes the preparation, characterization, and evaluation of gold nanoparticles modified with chitosan (Chi/AuNPs) as a reducing and stabilizing agent with efficient antimicrobial effects. In recent years, the development of an efficient and ecofriendly method for synthesizing metal nanoparticles has attracted a lot of interest in the field of nanotechnology. Colloidal gold nanoparticles (AuNPs) were prepared by the chemical reduction of gold ions in the presence of chitosan (Chi), giving Chi/AuNPs. The characterization of Chi/AuNPs was carried out by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), and X-ray diffraction (XRD). Chi/AuNPs appeared spherical and monodispersed, with a diameter ranging between 20 to 120 nm. The synergistic effects of AuNPs and Chi led to the disruption of bacterial membranes. The maximum inhibitory impact was seen against P. aeruginosa at 500 µg/mL, with a zone of inhibition diameter of 26 ± 1.8 mm, whereas the least inhibitory effect was reported for S. aureus, with a zone of inhibition diameter of 16 ± 2.1 mm at the highest dose tested. Moreover, Chi/AuNPs exhibited antifungal activity toward Candida albicans when the MIC was 62.5 µg/mL. Cell viability and proliferation of the developed nanocomposite were evaluated using a sulphorhodamine B (SRB) assay with a half inhibitory concentration (IC50) of 111.1 µg/mL. Moreover, the in vitro wound-healing model revealed that the Chi/AuNP dressing provides a relatively rapid and efficacious wound-healing ability, making the obtained nanocomposite a promising candidate for the development of improved bandage materials.

Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria

“…Antimicrobial nanoparticles have numerous potential benefits over traditional antibiotics, including several mechanisms of action on bacteria, easy and low-cost production, and great stability [22]. In vitro and in vivo studies have shown that antibacterial nanoparticles can suppress infection [23,24]. Furthermore, antimicrobial nanoparticles may harm bacteria in a variety of ways, making it harder for bacteria to acquire resistance [25].…”

Molecular Docking and Efficacy of Aloe vera Gel Based on Chitosan Nanoparticles against Helicobacter pylori and Its Antioxidant and Anti-Inflammatory Activities

“…Nanoparticles (NPs) have played and still play an important role in many medical, industrial, agricultural, environmental, and food fields [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. From the applied NPs, chitosan nanoparticles (CSNPs) have been lately used at large scales for medical applications such as drug carriers and cosmetics [ 24 ].…”

Green Synthesis and Antibacterial Activity of Ag/Fe2O3 Nanocomposite Using Buddleja lindleyana Extract

“…Leaf extract-mediated synthesis of nanomaterials is more eco-friendly and economical than other methods of synthesis such as chemical reduction and physical methods [ 5 , 6 , 7 ]. Due to the growing demand for ecologically friendly material synthesis techniques, the biosynthesis of nanomaterials has gained attention as an emerging feature of the interface of nanotechnology and biotechnology [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. The biosynthesis of inorganic materials, particularly metal nanoparticles, utilizing microbes and plants has received a lot of attention [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ].…”