Synthesis of Calcium Phosphate Composite Organogels by Using Emulsion Method for Dentine Occlusion Materials

Nopteeranupharp, C; Akkarachaneeyakorn, Khrongkhwan; Songsasaen, A

doi:10.1088/1757-899x/317/1/012027

Cited by 2 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Though in few reports, variation in size of nanoparticles was reported due to differences in frequency of stirring and pH of the suspension [24]. The zeta potential reported here for the nanoparticles was similar to that reported by others for calcium phosphate nanoparticles [40][41][42].…”

Section: Discussionsupporting

confidence: 88%

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever

Singh

Saxena

Singh

et al. 2022

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. The conventional adjuvants used in vaccines have limitations like induction of an imbalanced Th1 and Th2 immune response. To overcome this limitation, novel adjuvants and newer forms of existing adjuvants like calcium phosphate nanoparticles are being tested. Hypothesis/Gap Statement. Calcium phosphate adjuvanted outer membrane proteins vaccine may work as an efficient, safe and cost effective vaccine against Salmonella Typhi. Aim. Our goals were to evaluate the potential of calcium phosphate nanoparticles as an adjuvant using outer membrane proteins (Omps) of Salmonella Typhi as antigens for immune response, with montanide (commercially available adjuvant) as control, and its toxicity in rats. Methodology. Calcium phosphate adjuvanted outer membrane proteins nanoparticles were synthesized and characterized. The efficacy of vaccine formulation in mice and toxicity assay were carried out in rats. Results. The calcium phosphate nanoparticles varying in size between 20–50 nm had entrapment efficiency of 41.5% and loading capacity of 54%. The calcium phosphate nanoparticle-Omps vaccine formulation (nanoparticle-Omps) induced a strong humoral immune response, which was significantly higher than the control group for the entire period of study. In the montanide-Omps group the initial very high immune response declined steeply and then remained steady. The immune response induced by nanoparticle-Omps did not change appreciably. The cell mediated immune response as measured by lymphocyte proliferation assay and delayed type hypersensitivity test showed a higher response (P<0.01) for the nanoparticles-Omps group as compared to montanide-Omps group. The bacterial clearance assay also showed higher clearance in the nanoparticles-Omps group as compared to montanide-Omps group (approx 1.4%). The toxicity analysis in rats showed no difference in the values of toxicity biomarkers and blood chemistry parameters, revealing vaccine formulation was non-toxic in rats. Conclusion. Calcium phosphate nanoparticles as adjuvant in vaccines is safe, have good encapsulation and loading capacity and induce a strong cell mediated, humoral and protective immune response.

show abstract

Section: Discussionsupporting

confidence: 88%

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever

Singh

Saxena

Singh

et al. 2022

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Because the gel is synthesized for use in dentistry and directly onto the body, it is important that the employed substances are not toxic. Herein, oleic fatty acid was used as an oil phase and gelatin was employed as an additive for gel formation and template to determine the direction and size of the particles together with surfactant since it is a substance found in general cooking and used as an effective drug transporter in the body [3].The surfactant used in this work was Tween 20. Since oleic fatty acid was as an external phase, surfactant used in the system should have an HLB value of 17.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Calcium Phosphate Composite Bioactive Oleic Acid-Based Gels and its Application for Dentine Tubule Occlusion

Harnkla

Chienthavorn

Akkarachaneeyakorn

2020

MSF

View full text Add to dashboard Cite

Dentine hypersensitivity (DH) is a common issue among humans caused by the erosion of tooth enamel as a result of chewing and brushing incorrectly. The most effective treatment method is occluding the exposed dentine tubules. Because calcium phosphate is an important mineral and the most common mineral for dentine and orthopaedic application, this study focuses on the synthesis of bioactive gels containing calcium phosphate nanoparticles. The synthesis is performed using water-in-oil emulsion and characterized by the use of TGA, Rheological, TEM, and SEM techniques. This method used oleic fatty acids as an external phase and Tween 20 as surfactant to form water-in-oil nanodroplets. Gelatine was used to form the substance into a gel and increase its stability. The resulting nanoparticles had the same spherical shape and a similar size. Therefore, the varying amounts of gelatin do not affect the particle size. However, viscoelastic properties of gels were found to be changed. The ability of the gel for dentine tubule occlusion was investigated using SEM techniques. After 1 day, it was found that the gels can occlude dentine tubules.

show abstract

Synthesis of Calcium Phosphate Composite Organogels by Using Emulsion Method for Dentine Occlusion Materials

Cited by 2 publications

References 8 publications

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever

Synthesis of Calcium Phosphate Composite Bioactive Oleic Acid-Based Gels and its Application for Dentine Tubule Occlusion

Contact Info

Product

Resources

About