Synthesis of C C, C N coupled novel substituted dibutyl benzothiazepinone derivatives and evaluation of their thrombin inhibitory activity

Baburajeev, C. P.; Mohan, Chakrabhavi Dhananjaya; Pandey, Vijay; Rangappa, Shobith; Naveen, S.; Kalash, Leen; Sannaningaiah, Devaraja; Bender, Andreas; Lobie, Peter E.; Rangappa, Kanchugarakoppal S.; Basappa, Basappa

doi:10.1016/j.bioorg.2019.03.004

Cited by 6 publications

(1 citation statement)

References 45 publications

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“…Irreversible inhibitors that inhibit the thrombin activity completely at the tested concentration will have ∆RFU = 0 and will show 100% relative inhibition. The inhibition (%) was calculated by (slope of enzyme control − slope of fraction)/slope of enzyme control × 100% [53].…”

Section: Thrombin Inhibitory Activity Assaymentioning

confidence: 99%

Based on Multi-Activity Integrated Strategy to Screening, Characterization and Quantification of Bioactive Compounds from Red Wine

Gao

Wang

et al. 2021

Molecules

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According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.

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Section: Thrombin Inhibitory Activity Assaymentioning

confidence: 99%