Synthesis of bovine serum albumin-protected high fluorescence Pt₁₆-nanoclusters and their application to detect sulfide ions in solutions

Abstract

Highly fluorescent (quantum yield, QY = 17%) Pt16-nanoclusters (Pt16-NCs@BSA) have been prepared via a one-step ultrasonic-assistance method by using cheap and easily available ascorbic acid as reductant and bovine serum albumin (BSA) as a stabilizing agent in aqueous solution. The fluorescence properties of the Pt-NCs@BSA can be easily controlled by optimizing conditions, and the products are extremely stable and could be used for the detection of sulfide ions (S(2-)) in solutions as a specific luminescence sensor. The present synthesis method is performed in one step, being cost-effective with a particularly short reaction time, which could be extended to the synthesis of other kinds of protein-protected Pt-NCs.

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

“…Special efforts are being addressed towards the synthesis of platinum NCs (PtNCs). PtNCs show lower toxicity than QDs [19] and templates such as proteins, DNA, polymers and dendrimers have been investigated for the synthesis of water soluble PtNCs [20][21][22][23][24]. However, major drawbacks of big scaffolds are the difficulties both to remove the remaining free templates from the NCs in solution and to isolate the NCs from the big scaffold.…”

White Light Emitting Gadolinium Oxide Nanoclusters for In‐vitro Bio‐imaging

“…Moreover, due to the small size and steric ChemistrySelect protection of Gd 2 O 3 by BSA, it could be safely utilized in the biological system and retained for longer. [29,40] The BSA protein is well known to serve as a template for controlled nucleation and grain growth of nanoclusters imparting colloidal stability, [41] and the commercially available ascorbic acid was used as a reductant, which is non-toxic and biodegradable [42] in nature. It has been observed that fluorescence intensity in longer wavelengths (green, red) increases (ESI-Figure S1) with incubation time (up to 72 hrs) due to the formation of a larger nanocluster, which is in good harmony with other published articles.…”

Gold–Platinum Bimetallic Nanoclusters for Oxidase-like Catalysis

“…The intensities of binding energy for Au(0) and Au(I) reveal that their ratio in particles was 66.31 and 33.69%, respectively, confirming the predominance of Au(0), while that of Pt in NCs (Figure 5D) shows a strong peak at 72.8 eV for Pt 4f7/2 , which is assigned to Pt(I). 50 The results show that the binding energies of Au (Au 4f7/2 , 84.4 eV) and Pt (Pt 4f7/2 , 72.8 eV) in Au−PtNCs-GMP are obviously different from those of Au 4f7/2 (84.0 eV) 51 and Pt 4f7/2 (71.6 eV) in bulk, 50 respectively. Therefore, the modified electronic structures of metal enhance the catalytic properties of Au−PtNCs-GMP.…”