Synthesis of Biotinylated Ferrocene, and Its Application as a Redox Probe for Electrochemical Immunosensor

Abstract

The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene through covalent bonding was studied. Synthesis and structure identification of Biotinylated 1 -(-2-aminoethyl) ferrocene were performed by 1 H-NMR, 13 C-NMR and MS. The ultimate goal of this paper is to develop derivatives of ferroceneservingas an electrochemical probe that would enhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidin layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry (DPV) method and underlined a variation of redox properties upon the antigen interaction. The introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good sensitivity of the biosensor with a detection limit of 0.16 pg.mL -1 .