Synthesis of bioactive oligosaccharides and their potential health benefits

Chen, Yihan; Wen, Yuxi; Zhu, Yingying; Chen, Zhengxin; Mu, Wanmeng; Zhao, Chao

doi:10.1080/10408398.2023.2222805

Cited by 4 publications

(4 citation statements)

References 111 publications

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“…UV/H 2 O 2 treatment effectively degraded the polysaccharides, resulting in the formation of low molecular weight polysaccharides. This reduction in molecular weight potentially improved their bioavailability, solubility, and cellular uptake, enhancing their interaction with target cells and increasing their bioactivity [ 50 , 51 ]. Besides, UV/H 2 O 2 treatment did not significantly alter the functional groups in DSFPs, ensuring that the essential chemical structures responsible for their biological effects remained intact.…”

Section: Resultsmentioning

confidence: 99%

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Yao

Jiang

et al. 2023

Marine Drugs

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The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H2O2) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H2O2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H2O2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H2O2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H2O2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides.

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Section: Resultsmentioning

confidence: 99%

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Yao

Jiang

et al. 2023

Marine Drugs

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“…The MOs have an enormous molecular weight that cannot be directly utilized by cell, but can be catabolized by gut microbiota, then producing SCFAs to respond to various life activities, such as immune regulation and glucose homeostasis ( Zhu et al, 2020 ; Zhang et al, 2021 ; Chen et al, 2023 ). SCFAs are the main products of prebiotic fermentation, and the production of these metabolites depends on both dietary fiber types and gut microbiota composition ( Bridgman et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…This process highlights A. muciniphila's critical role in shaping the gut microbiota of neonatal piglets, underscoring its evolutionary adaptability and ecological significance, similar to its beneficial functions in human infants. The MOs have an enormous molecular weight that cannot be directly utilized by cell, but can be catabolized by gut microbiota, then producing SCFAs to respond to various life activities, such as immune regulation and glucose homeostasis (Zhu et al, 2020;Zhang et al, 2021;Chen et al, 2023). SCFAs are the main products of prebiotic fermentation, and the production of these metabolites depends on both dietary fiber types and gut microbiota composition (Bridgman et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Utilization of Ningxiang pig milk oligosaccharides by Akkermansia muciniphila in vitro fermentation: enhancing neonatal piglet survival

Zhang,

Wu,

Kang

et al. 2024

Front. Microbiol.

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Akkermansia muciniphila (A. muciniphila), an intestinal symbiont residing in the mucosal layer, shows promise as a probiotic. Our previous study found that the abundance of A. muciniphila was significantly higher in Ningxiang suckling piglets compared to other breeds, suggesting that early breast milk may play a crucial role. This study examines A. muciniphila’s ability to utilize Ningxiang pig milk oligosaccharides. We discovered that A. muciniphila can thrive on both Ningxiang pig colostrum and purified pig milk oligosaccharides. Genetic analysis has shown that A. muciniphila harbors essential glycan-degrading enzymes, enabling it to effectively break down a broad spectrum of oligosaccharides. Our findings demonstrate that A. muciniphila can degrade pig milk oligosaccharides structures such as 3′-FL, 3′-SL, LNT, and LNnT, producing short-chain fatty acids in the process. The hydrolysis of these host-derived glycan structures enhances A. muciniphila’s symbiotic interactions with other beneficial gut bacteria, contributing to a dynamic microbial ecological network. The capability of A. muciniphila to utilize pig milk oligosaccharides allows it to establish itself in the intestines of newborn piglets, effectively colonizing the mucosal layer early in life. This early colonization is key in supporting both mucosal and metabolic health, which is critical for enhancing piglet survival during lactation.

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“…These glycosyltransferases are constantly identified from different microorganisms such as WbsJ (encoding α1–2-fucosyltransferase from Escherichia coli O128:B12), and SAMT (encoding α1–2-fucosyltransferase from Azospirillum lipoferum ). Moreover, these lactose-core HMOs can be synthesized in engineered bacteria by introducing glycosyltransferases into GDP-fucose or CMP-Neu5Ac pathway . Lacto- N -triose II (LNTri II, GlcNAcβ1–3Galβ1–4Glc) is the core of more complex HMO derivatives and the direct precursors of lacto- N -tetraose (LNT) and lacto- N -neotetraose (LNnT), and LNT (Galβ1–3GlcNAcβ1–3Galβ1–4Glc) and LNnT (Galβ1–4GlcNAcβ1–3Galβ1–4Glc) can be synthesized by overexpressing β1,3/4-galactosyltransferase genes in engineered bacteria .…”

Section: Structural Categories and Synthesis Of Hmosmentioning

confidence: 99%

Recent Progress in Human Milk Oligosaccharides and Its Antiviral Efficacy

Chen,

Zhu

et al. 2024

J. Agric. Food Chem.

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Gastrointestinal (GI)-associated viruses, including rotavirus (RV), norovirus (NV), and enterovirus, usually invade host cells, transmit, and mutate their genetic information, resulting in influenza-like symptoms, acute gastroenteritis, encephalitis, or even death. The unique structures of human milk oligosaccharides (HMOs) enable them to shape the gut microbial diversity and endogenous immune system of human infants. Growing evidence suggests that HMOs can enhance host resistance to GI-associated viruses but without a systematic summary to review the mechanism. The present review examines the lactose-and neutral-core HMOs and their antiviral effects in the host. The potential negative impacts of enterovirus 71 (EV-A71) and other GI viruses on children are extensive and include neurological sequelae, neurodevelopmental retardation, and cognitive decline. However, the differences in the binding affinity of HMOs for GI viruses are vast. Hence, elucidating the mechanisms and positive effects of HMOs against different viruses may facilitate the development of novel HMO derived oligosaccharides.

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Synthesis of bioactive oligosaccharides and their potential health benefits

Cited by 4 publications

References 111 publications

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment

Utilization of Ningxiang pig milk oligosaccharides by Akkermansia muciniphila in vitro fermentation: enhancing neonatal piglet survival

Recent Progress in Human Milk Oligosaccharides and Its Antiviral Efficacy

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