Synthesis of amphiphilic triblock copolymers and application for morphology control of calcium carbonate crystals

Yang, Hengrui; Su, Yunlan; Zhu, Haijin; Zhu, Hui; Xie, Baoquan; Zhao, Ying; Chen, Yongming; Wang, Dujin

doi:10.1016/j.polymer.2007.05.073

Cited by 29 publications

(21 citation statements)

References 16 publications

(9 reference statements)

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“…Previously, we demonstrated a convenient and flexible self‐assembled synthetic strategy for producing supramolecular nanoparticle [ 18 ] (SMNP) vectors by mixing three molecular building blocks, i.e., β ‐cyclodextrin (CD)‐grafted branched polyethyleneimine (CD‐PEI), adamantane (Ad)‐grafted polyamidoamine dendrimer (Ad‐PAMAM), and Ad‐grafted poly(ethylene glycol) (Ad‐PEG). The multivalent Ad/CD molecular recognition allows modular control over the sizes, surface chemistry, and payloads of SMNP vectors, promising a diversity of imaging [ 18,19 ] and therapeutic applications. [ 20 ] We have shown that this self‐assembly strategy can be utilized for combinatorial formulation and screening of SMNPs to optimize formulations with significantly improved delivery performance.…”

Section: Methodsmentioning

confidence: 99%

“…To prepare for the in vitro study, the B16 mouse melanoma cell line (no RS1 gene expression) was employed as a model system for optimization. Our self‐assembly strategy [ 18 ] enables precise control over two synthetic variables—(i) Ad‐PAMAM/CD‐PEI ratios, and (ii) the coverage of a membrane penetration ligand, TAT. [ 20c ] Through small‐scale combinatorial screenings, optimal performances were identified for the formulations of Cas9/sgRNA‐plasmid⊂SMNPs and Donor‐RS1/GFP‐plasmid⊂SMNPs according to their performances for inducing CRISPR/Cas9‐mediated insertion and deletion events (indels) and GFP transfection, respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X‐Linked Juvenile Retinoschisis

et al. 2020

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The homology-independent targeted integration (HITI) strategy enables effective CRISPR/Cas9-mediated knockin of therapeutic genes in nondividing cells in vivo, promising general therapeutic solutions for treating genetic diseases like Xlinked juvenile retinoschisis. Herein, supramolecular nanoparticle (SMNP) vectors are used for codelivery of two DNA plasmids-CRISPR-Cas9 genome-editing system and a therapeutic gene, Retinoschisin 1 (RS1)-enabling clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) knockin of the RS1 gene with HITI. Through small-scale combinatorial screenings, two SMNP vectors, with Cas9 and single guide RNA (sgRNA)plasmid in one and Donor-RS1 and green fluorescent protein (GFP)-plasmid in the other, with optimal delivery performances are identified. These SMNP vectors are then employed for CRISPR/Cas9 knockin of RS1/GFP genes into the mouse Rosa26 safe-harbor site in vitro and in vivo. The in vivo study involves intravitreally injecting the two SMNP vectors into the mouse eyes, followed by repeated ocular imaging by fundus camera and optical coherence tomography, and pathological and molecular analyses of the harvested retina tissues. Mice ocular organs retain their anatomical integrity, a single-copy 3.0-kb RS1/GFP gene is precisely integrated into the Rosa26 site in the retinas, and the integrated RS1/GFP gene is expressed in the retinas, demonstrating CRISPR/Cas9 knockin of RS1/GFP gene.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X‐Linked Juvenile Retinoschisis

et al. 2020

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“…[149] Along the idea of non-covalent formulation approaches, we have previously demonstrated a convenient, flexible, and modular self-assembly approach for the preparation of supramolecular nanoparticle (SNP) vectors from a small collection of molecular building blocks through a multivalent molecular recognition based on adamantane (Ad) and β-cyclodextrin (CD) motifs (see Chapter 2.1.). [150] In order to further expand the utility of SNP vectors for delivery of an active anti-cancer drug (i.e., doxorubicin, Dox), we test the feasibility of producing Doxencapsulated SNPs (Dox⊂SNPs) through self-assembly of SNP molecular building blocks in the presence of free Dox molecules. It is well-known that Dox is an intrinsically fluorescent compound whose photophysical properties are highly susceptible to its surrounding environment.…”

Section: Gene Delivery Performance Of Tat/rgd-dna⊂snpsmentioning

confidence: 99%

Supramolecular Nanoparticles for Molecular Diagnostics and Therapeutics

Chen

Garcia

Wang

et al. 2012

Supramolecular Chemistry

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Chapter 1. BACKGROUND …………………………………………………………………. Chapter 2. SUPRAMOLECULAR NANOPARTICLES (SNPs) ………………………….. My graduate study at UCLA was a precious, unique and memorable experience. All the challenges and experiences had happened made me grow stronger and wiser. Moreover, the acquired experiences and professional skills help me prepare myself to face any future challenges in my life and the next career. I could not have completed my Ph.D. journey at UCLA without the guidance, support, and encouragement of many people. Especially, I would like to thank my advisor, Prof. Hsian-Rong Tseng, for his great mentorship. I will never forget the lessons he taught me throughout the course of my research. His insight, guidance, inspiration, and support not only trained me to become an independent research scientist but also helped me to develop my own strength. Dr. Tseng is not just an advisor but a father figure to me. Just like a father, knowing that I could be better, he always has higher expectations on me. I am particularly grateful to the way he mentors me, which make me to accomplish lots of fruitful results and motivate me to achieve many ambitious goals. I have been fortunate to be surrounding by extremely hardworking, super friendly, and remarkably talented group members in Dr. Tseng's lab. In my first year of graduate training, I was assigned to work with two outstanding postdoc fellows (now are professors in China), Dr.

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“…Block copolymer-based assemblies have been extensively used in the biomimetic formation of different hybrid materials [34][35][36][37][38][39], but hardly ever in directing the formation of calcium carbonate [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic synthesis of calcium carbonate bilayers interfaced by a diblock copolymer template

Goos

Dey

et al. 2012

Zeitschrift Für Kristallographie - Crystalline Materials

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Abstract. The synthesis of a new class of hybrid materials with two differently oriented layers of calcite at adjacent sides of an organic template is demonstrated. A Langmuir monolayer of the amphiphilic block copolymer poly(butyl acrylate)-b-poly(hydroxypropyl acrylate) directs the formation of a first CaCO 3 phase through interaction with the hydrophilic poly(hydroxypropyl acrylate) blocks. After partial hydrolysis of the hydrophobic poly(butyl acrylate) segments a second CaCO 3 phase is formed on the monolayer associated to the first mineral phase. Thus, bilayered CaCO 3 -based hybrid materials are obtained with two differently oriented calcite phases at opposite sides of the polymer film. By using DNA as an additive the crystal orientation in the second layer can be modified.

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Synthesis of amphiphilic triblock copolymers and application for morphology control of calcium carbonate crystals

Cited by 29 publications

References 16 publications

Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X‐Linked Juvenile Retinoschisis

Dual Supramolecular Nanoparticle Vectors Enable CRISPR/Cas9‐Mediated Knockin of Retinoschisin 1 Gene—A Potential Nonviral Therapeutic Solution for X‐Linked Juvenile Retinoschisis

Supramolecular Nanoparticles for Molecular Diagnostics and Therapeutics

Biomimetic synthesis of calcium carbonate bilayers interfaced by a diblock copolymer template

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