Synthesis of Amino Acid Capped Silver Nanoparticles, Characterization, and Biological Application

Abstract

In the present work, chemical reduction method has been used to synthesize silver nanoparticles using glycine amino acid as a capping agent in different concentrations. The synthesis is optimized for the time by observing the reaction at 30, 60, 120, 180, 240, 300 and 360 minutes. The optimum time for this synthesis is 240 minuters. after this time there was no change in absorbance. Ultraviolet-visible spectroscopy and Dynamic light scattering (DLS) techniques are used to characterize these nanoparticles. The surface plasma resonance (SPR) peak in the range between 400-427 nm confirmed the synthesis of silver nanoparticles. These silver nanoparticles are 50-60 nm in diameter. The antibacterial activity of synthesized nanoparticles is evaluated against E. coli and S. aureus. Nanoparticles show a bactericidal effect on Gram +ve and Gram -ve bacteria. As the concentration of glycine increased, the diameter of the zone of inhibition against E. coli as well as S. aureus also increased.