Synthesis of Alloplasmic Brassica campestris as a New Source of Cytoplasmic Male Sterility

Abstract

Cytoplasmic male, steriles of Brassica eampestris ssp. oleifera var, brown sarson -p.-ere obtained in BC5, generation progenies follo-^-ing the repeated backcrossing of the synthetic alloploid B. oxyrrhina (2n = 18, OO) X B. campestris (2n = 20, AA), Allopiasmic B. campestris plants resemble B. campestris 111 morpholog)-and grou-th pattern and do not e,Khibit any trait of 8. oxyrrhina. Ho-R-ever, the leaves are mildly chlorophyll deficient in the earlier stages and turn green at late stages in the development. The reproducti\-£ parts do not show an}-abnormalities except that the anthers are snia.ller and slendf-er than the normal ones and are non-dehiscent, containing only sterile pollen.

Production of Intergeneric Hybrids Between Sinapis alba and Brassica carinata

“…Hybridization between cultivated species of Brassica and related genera is an important means for introgression of desirable genes to the cultivated forms and for synthesizing cms lines through cytoplasmic substitution (Prakash and Chopra 1988). A rational approach to obtain sexual hybrids between given genotypes would require that the barriers to hybridization be identified and overcome systematically.…”

Heterosis and Its Utilization

“…Male sterility resulted from the alloplasmic combination of B. oxyrrhina cytoplasm with the nuclear genomes of B. campestris (Prakash and Chopra 1988) and B. juncea (Prakash and Chopra 1990). The synthetic alloploid between B. oxyrrhina (00; 2n = 18) and B. campestris spp.…”

Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis

“…In several Brassica crop species ( B. rapa , B. juncea and B. napus ), a number of male sterile cytoplasms from related Brassica species have been introduced aggressively through interspecific and intergeneric hybridization or protoplast fusion followed by successive backcrossing: Diplotaxis muralis (Hinata and Konno 1979), Brassica tournefortii (Mathias 1985), Brassica oxyrrhina (Prakash and Chopra 1988), Diplotaxis siifolia (Rao et al 1994), Trachystoma ballii (Kirti et al 1995), Eruca sativa (Matsuzawa et al 1999), Erucastrum canariense (Prakash et al 1998), Diplotaxis catholica (Pathania et al 2003), Enarthrocarpus lyratus (Deol et al 2003), Diplotaxis erucoides (Bhat et al 2006) and Diplotaxis berthautii (Bhat et al 2008). On the other hand, the cytoplasm of Moricandia arvensis , which was first reported as a C 3 -C 4 intermediate species, has also been introduced to B. juncea (Prakash et al 1998) and R. sativus (Bang et al 2002).…”