Healthcare tracking supports early diagnosis of illnesses, realtime tracking of the impacts of therapy and treatment, and tracking of cases of human health. Based on this, real-time tracking of sweat loss provides an easy, convenient, and noninvasive approach to the early diagnosis of physical illness in individuals. To date, copper oxide (CuO) as a nanostructured semiconductor metal-oxide is regarded as a hopefully p-type sensing material. The corresponding sweat rate monitoring systems were fabricated using a repeatable and cost-effective SILAR system using a cellulose acetatebased organic substrate. To provide a practical application, we investigated the working efficiency of Al:CuO under room conditions since most clinical and healthcare industries operate under ambient temperature. Fabricated flexible devices immediately respond to the implementation of the sweat solution and reach a steady-state value in a short time. It can be obtained from experimental results that the sweat-loss monitoring performances of CuO-based devices can be enhanced by employing Aldoping. The increment in the sensing efficacy was interpreted in terms of structural and morphological characterization and electrical data. Our designed flexible thin film-based system can be used in conjunction with a customized wearable, healthcare industry integrated, low-cost fabrication source.